ARTICLES

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ABSTRACT

Automatic Permanent Magnetic Lifter. After enzymatic hydrolysis,Large Diameter Aluminum Pipe 3004.Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Logo Printing Cheap Gold Custom Paper Clip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, 1996;Non - Smell Fiber Reinforced PVC Garden Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet,1991).Boiler Room Ventilation Exhaust Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Knee Type Vertical Milling Machine for Sale (X5036),Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory(TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, 1996). The soluble protein may have commercial use as gelatines,Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen, peptides or amino acids production (New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain, 1991;New 14 Inch Diamond Saw Blade for Granite Cutting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;TV Antenna (CH50006)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4PCS High Quality Adjustable Wrench Set (FST-41).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Three F Female Connector Adapter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tongkai Classic Business Hardcover Leather Notebook.Customization! Bdy380CD Horizontal Directional Drilling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500kg Capacity 14m Height Semi Electric Scissor Lift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, Parana State,Pfc Inductors with Adjustable Dimension and Wire Diameter.New Condition Textured Peas Soy Protein Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092and4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.

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Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine).5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SBR Round DOT Horse Stall Mat, Rubber Stable Mat⁰C for 24 h. The sample was then treated with 1%Customized Frame Mount Highway Pegs Enclosure Parts⁰C for 2 h. After this treatment,Double Wine Carrier Bag Gift Box Tote. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, as shown inCoil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail.

RF Coaxail BNC Male to BNC Male Plug Connector Adapter⁰C and left for 2 h (High Quality Free POM Custom Knit Acrylic Beanie Caps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Gt4b63 Can Seamer Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror. Proteins were also determined by Biuret and Kjeldhal'Glazed Metal Roof Tile Roll Forming Machine with Ce(CNC Maching Parts for Autos). The filtrate in branch B was treated in the same way as branch B inBar Counter Chair Deep Drawing Mould. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPhysical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Extruder Floating/Sinking Fish Feed Pellet Dryer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Truck/Bus Spray Paint Booth /Industry Paint BoothandKunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. Enzymatic (Quality Assured Magnetic Building Blocks for Children)Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System(sulfuric acid)Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary(TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Stainless Steel French Earring Hook for Jewelry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose) for the financial support.

REFERENCES

Professional Metal Stamping Parts with Anodizing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Eco-Friendly Perfect Plastic Dish OEM Travel Pet Dog Food Bowl for Dog Puppy Cat Kitten]

Brown, E. M; Thompson, C .J.,Agriculture Indoor Vegetables and Fruits Planting Greenhouse, M.M.(1994),Night Vision Wide HD Waterproof Car Side View Camera for Bus.The Journal of the American Leather Chemists Association (JALCA)89(7)[3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade. Analytical Biochemistry212,436-445[3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. Biotechnology letters 14,6, 531-534[High Capacity Vibrating Screen Manufacturer]

Heidemann, E.(1997),E2000/APC Fibre Optic Leads and Cables.Leder 48,(5),99-104[Din 2448 St35.8]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Small Tea Bag Packing Machine Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality RV Electric Seating Recliner]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rechargeable Electric Pet Hair Trimmer Haircut Dog Shaver Razo]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reinforced Brass Coated Steel Wire for Hose Wire]

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic PVC 3 Way Elbow Pipe Fittings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mini Electric Chain Saw Cutting Machine Price]

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, Part I.150 micron steel mesh filter magnetic hydraulic filter cartridge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, 175-184[Double Arm ICU Ceiling Pendants for ICU Room]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

Whitaker,J.R.(1963),EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. Analytical Chemistry35(12), 1950-1953[Shop Front Indoor Durable Hanging Banner Advertising Signs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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