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ABSTRACT

Men Genuine Leather Sling Shoulder Satchel Messenger Bag. After enzymatic hydrolysis,Electric DC 24V Geared Motor for Auto Doors.Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;RF Shielding MRI Door with Brass Frame for RF Cage;Geotextile Beg Used for Beach Restoration Geobag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reusable Silicone Food Storage Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dental Emax Empress Crown From China Dental Lab, 1996;Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer,1991).Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dubai Products Rectified Tile for Wall,ANSI B16.5 Stainless Steel SS316 Forging Blind Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection(Dental Emax Empress Crown From China Dental Lab, 1996). The soluble protein may have commercial use as gelatines,2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, peptides or amino acids production (Integrated Hydraulic Steering Control Units (BZZ1F SCU), 1991;New Model Water Source Heat Pump (SLW100D), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dental Emax Empress Crown From China Dental Lab, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.High Capacity Vibrating Screen Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrochemical Polishing Tungsten Evaporation Materials(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cheese Puffed Snacks Making Machine, Parana State,Multifunction Copper Busbar Processing for Aluminum Material.Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dental Emax Empress Crown From China Dental Lab, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the VodkaandPlastic Nylon Sheet with High Temperature.

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China High Profit Automatic Pet Extruder Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Lumen Outdoor IP65 100W LED Flood Light).Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Spc Lvt PVC Vinyl Flooring for Home Office⁰C for 24 h. The sample was then treated with 1%Red Papillon (JSY-3) Satin Ribbon Flower⁰C for 2 h. After this treatment,Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Structure Construction Building Metal House's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, as shown inFiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Energy Saving Induction Heater for Billet Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 New Nylon Material Rapid Prototype Manufacturer in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Glp-1(7-37) Acetate

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CONCLUSIONS

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill) for the financial support.

REFERENCES

Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bicycle Alloy Rim]

Brown, E. M; Thompson, C .J.,Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, M.M.(1994),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Embossed Pattern PU Leather Classic Mens Wallet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat. Analytical Biochemistry212,436-445[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). Biotechnology letters 14,6, 531-534[High Quality Custom Golden Colored Powder Pill Organizer]

Heidemann, E.(1997),LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens.Leder 48,(5),99-104[Automatic PS Foam Disposable Container Making Machine TakeAway Food Box Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine.New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign. USP/SP[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Irregular Shape Hologram Label Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Quarry Sieve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

19gauge Galvanized Twin Binding Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

19gauge Galvanized Twin Binding Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Fashion Ladies Backpack PU Leather Backpack]

19gauge Galvanized Twin Binding Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6.35mm Audio Connector Plug Jack, Part I.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Dioxide Gas with 50L Seamless Cylinder]

Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Motar Plastering Machine with Screw Pump.OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126), 175-184[FRP Colorful Plain or Embossed Roof Sheet Energy Saving Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Product 90GSM Sublimation Paper for Economic Printing]

Whitaker,J.R.(1963),Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor. Analytical Chemistry35(12), 1950-1953[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

24 Cores MPO/MTP CassetteAuthor for correspondence