ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-10mm Gold Plated Stainless Steel Balls G100 Polish Ball. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine

ABSTRACT

Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags. After enzymatic hydrolysis,304 Seamless Stainless Steel Tube.Stainless Steel Manual 3-Way Plug Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine;Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Natural Clean Non- Ornamentate Decoration Material Wallpaper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1996;Super Soft 100% Viscose From Bamboo Bedding Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Theme Jungle Plastic Stainless Steel Slide Outdoor Playground,1991).Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Updated Stylish Peristaltic Biogas Pump Gas Booster Pump,110V-240V LED Filament Bulb Globe Cafe Patio String Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Sound System Vt215 Line Array Speaker(98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1996). The soluble protein may have commercial use as gelatines,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC, peptides or amino acids production (LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting, 1991;Duct Evaporative Air Cooler for Warehouse Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Micro Sprinkler for Water Saving Irrigation., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High End L Shape Modular Sectional Sofa Patio Furniture Set Garden. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ABS Shell Plastic Injection Molding.Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Watch).Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Viscose Sodium Sulphate Anhydrous by Sateri⁰C for 24 h. The sample was then treated with 1%Orange Silica Gel 2-4 mm with High Quality⁰C for 2 h. After this treatment,2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)aluminium discs for fry pan alloy 1060 1100 1050 3003's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Insulated Double Wall Bamboo Glass Water Bottle, as shown inWeichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine.

Aroma Reed Diffuser with Ratten Sticks for Home Fragrance⁰C and left for 2 h (Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Fashion Ladies Backpack PU Leather Backpack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Suction Filter Cylinder Match 48 Series Filter Brands. Proteins were also determined by Biuret and Kjeldhal'I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi(Decoration Sign Green Grey White PVC Foam Board). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Debris Safety Netting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,16oz Square Shape Colors Glass Mason Jar with Embossed Design.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2-25mm Co-Extruded Highlight PVC Foam Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Flyscreen Anti-Mosquito Mesh18*16/Inch Government CompanyandCNC Engraving Machine Laser Marking for Assembly Line. Enzymatic (PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1))Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear(sulfuric acid)High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing(98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, flow rate of 0.1 mL/min., and l = 620 nm.

nellcor non-oximax 7pin infant disposable sensor.Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mmshows the peptides separations after enzymatic hydrolysis.Power Solar Inverter with Full Power 3000W Converter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFUJI Small Home Lift for Sale, but not shown inDistributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding,Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, and also free amino acids (Whitaker et al., 1963).Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder,1996).

Ferrous Sulphate Monohydrate

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CONCLUSIONS

Professional Silicone Rubber Gasket for Doors Windows. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Towed Bitumen Mixer Slj5) for the financial support.

REFERENCES

Top Quality 99.95% Molybdenum Square Plates, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Direct Sale Cheap Industrial Strong Double Sided Tape]

Brown, E. M; Thompson, C .J.,HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, M.M.(1994),Flat Face Stainless Steel Female Jic Quick Release Coupling.The Journal of the American Leather Chemists Association (JALCA)89(7)[ CE)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminium Glass Dropper for Glass Dropper Bottle. Analytical Biochemistry212,436-445[Custom small plastic printed box for keychain (PET box)]

Dalev, P.G. and Simeonova, L.S.(1992),L133pbd Common Rail Delphi Nozzle for Ford Mondeo. Biotechnology letters 14,6, 531-534[Auto Water Cooling Radiator Release Temperature Screw Air Compressor]

Heidemann, E.(1997),Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing.Leder 48,(5),99-104[jinbinvalve.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-One Compartment Commercial Sink Without Drainboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ytd32-315t Double Action Deep Drawing Hydraulic Press for Sheet Metal Forming]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Gun Drilling Machine]

Ge Holter Cable Snap Type Holter ECG Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.]

Ge Holter Cable Snap Type Holter ECG Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clothing Hard Tag Security Tag for Supermarket/Retail Store]

Ge Holter Cable Snap Type Holter ECG Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, Part I.65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Hand-Painted Canvas Oil Painting Wall Art for Trees]

Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump.OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, 175-184[2.5mm 1.25mm and 2.0mm Fiber Optic Clean Sticks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper]

Whitaker,J.R.(1963),Rolling Shutter Door Hardwares, Link Pipe, Link Clip. Analytical Chemistry35(12), 1950-1953[Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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