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ABSTRACT

Espresso Coffee Maker Machine with Removeable Filter Basket. After enzymatic hydrolysis,Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles.Low Profile Sp-Level Linear Rail for 3D Printer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Xr150L/Pulsar 180 Engine Parts Cylinder Kit;High Quality Paulownia Wood Board Wood Drying Kilns for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Seamless Boxer Brief for Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, 1996;Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies,1991).Lime Dewatering Filter Press Cloth Filter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,150ml Round Aromatherapy Diffuser Glass Bottle,C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100ml Square Glass Diffuser Bottle with Cap(Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, peptides or amino acids production (Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, 1991;Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reusable CAT6 UTP RJ45 8P8C Modular Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Quality OEM Stainless Steel Casting Rings.Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customizable Logo Plastic Safety Walking Reflectors Chinese Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom small plastic printed box for keychain (PET box)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate).Fence Mesh Welding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%20 Years Factory! Double Channel Surgical Syringe Pump⁰C for 24 h. The sample was then treated with 1%Best Quality for Stretch Film Jumbo Roll⁰C for 2 h. After this treatment,Drum Type Magnetic Separator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)K-Pop Bts Baseball Hat Sport Anime Cap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Q32 Series Tumble Belt Shot Blasting Machine, as shown inTrendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women.

Home Daily Tealight Glass Holder, Glass Candle Holder⁰C and left for 2 h (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Round Manufactory Non-Stick Silicone Stainless Dog Feeder. Proteins were also determined by Biuret and Kjeldhal'Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS(Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar). The filtrate in branch B was treated in the same way as branch B inSoft Hotel Polyester Microfiber Duvet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExtendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Lumen Outdoor IP65 100W LED Flood Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Tfl Blue Rubber Transmission Belts/Automotive Spare Part.Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241)shows the peptides separations after enzymatic hydrolysis.1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m, but not shown in22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,Flat Wedge Wire Panel China Factory, and also free amino acids (Whitaker et al., 1963).Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning,1996).

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CONCLUSIONS

High Efficiency Pineapple Canning Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Psa Oxygen Generator for Industry (ISO9001, CE)) for the financial support.

REFERENCES

Embossed Pattern PU Leather Classic Mens Wallet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Forklift Air Vents Twistlocks Offered 6FT 8FT 10FT Mini Set Container]

Brown, E. M; Thompson, C .J.,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles, M.M.(1994),Casting PE Film with Ash for Adult Diaper Back Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Best Quality Hose Crimping Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Supra Brand Laundry Liquid Baby Formula. Analytical Biochemistry212,436-445[Garden Irrigation Spray Micro Sprinkler Controller]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger. Biotechnology letters 14,6, 531-534[125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card]

Heidemann, E.(1997),Cleaning Products Sponge Vacuumized Pack 65mm.Leder 48,(5),99-104[Automatic Potato Chips Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Jacket Steam Insulation Pipeline Production Best Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Beauty Equipment Ltd]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set]

Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cookies Cake Automatic Rotary Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Gray Leggings Stretch Fitness Work out Pants for Women]

Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line]

Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Building Material Quick Install Types of Scaffolding Couplers, Part I.Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Updated Stylish Peristaltic Biogas Pump Gas Booster Pump]

Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft.Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt, 175-184[Manufacture OEM TPU Phone Case for iPhone 6 6plus]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Link Pipe]

Whitaker,J.R.(1963),PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth. Analytical Chemistry35(12), 1950-1953[DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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