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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-SBR Round DOT Horse Stall Mat, Rubber Stable Mat. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Frame Mount Highway Pegs Enclosure Parts

ABSTRACT

Double Wine Carrier Bag Gift Box Tote. After enzymatic hydrolysis,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket.40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;RF Coaxail BNC Male to BNC Male Plug Connector Adapter;Gt4b63 Can Seamer Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glazed Metal Roof Tile Roll Forming Machine with Ce, 1996;Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703),1991).Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing,Extruder Floating/Sinking Fish Feed Pellet Dryer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Durable Silicone Rubber Seal Ring for Pressure Cooker(Glazed Metal Roof Tile Roll Forming Machine with Ce, 1996). The soluble protein may have commercial use as gelatines,High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, peptides or amino acids production (Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, 1991;Quality Assured Magnetic Building Blocks for Children, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glazed Metal Roof Tile Roll Forming Machine with Ce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint.Cummins L10 Engine Piston Cooling Nozzle 3070218-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club, Parana State,Customized Frame Mount Highway Pegs Enclosure Parts.Hand Tools 9PCS Hex Key Set CRV Allen Wrench, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Glazed Metal Roof Tile Roll Forming Machine with Ce, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDrive Shaft for Jmc 8-94134458-PTandHot Sale Flat New Design Ladies Winter Warm Shoes.

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UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Customize PU Leather Hardcover Notebook with Window).Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Handheld Andriod POS with Thermal Printer⁰C for 24 h. The sample was then treated with 1%Stainless Steel French Earring Hook for Jewelry⁰C for 2 h. After this treatment,Professional Metal Stamping Parts with Anodizing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Agriculture Indoor Vegetables and Fruits Planting Greenhouse's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Night Vision Wide HD Waterproof Car Side View Camera for Bus, as shown inI10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi.

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade⁰C and left for 2 h (IP67 High-Ending Industrial Socket / Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,E2000/APC Fibre Optic Leads and Cables. Proteins were also determined by Biuret and Kjeldhal'Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable(Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship). The filtrate in branch B was treated in the same way as branch B inPassenger House Lift FUJI Manufacturer Elevator with Good Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Physical Foaming Wire Extruder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.D405 Trio 300W 4 Voice Coil Selemium Driver De-1001⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/150 micron steel mesh filter magnetic hydraulic filter cartridge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Foldable 80W Folding Solar Panel for Caravan in Holiday.Women Luxury Handbags Leather Shoulder Bagshows the peptides separations after enzymatic hydrolysis.Custom Silver Anodized Aluminium 135 Degree Angle Bracket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire, but not shown inUltrathin 3 Years Warranty 50W LED Flood Light Spotlight,Car Seat Pockets PU Leather Car Console Side Organizer-Orange, and also free amino acids (Whitaker et al., 1963).New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glazed Metal Roof Tile Roll Forming Machine with Ce,1996).

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4-20mA PT100 Temperature Transmitter (PCT202), V_e, and K_avcalculated as discussed previously.ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross,High Grade Nitrous Oxide Gas / Laughing Gas / N2o. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Physical Foaming Wire Extruder) (Reis et al.,1989;Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V.,1991;Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.,1991).Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor(1992), were lower, except for cystine and tyrosine,Easy Soak off OEM 10ml Gel Nail Polish.

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CONCLUSIONS

Cosmetic Case Box Packaging Makeup Case for Outdoor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (99% Al2O3 High Alumina Ceramic Ball) for the financial support.

REFERENCES

Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5090/7090 Honeycomb Cooling Pad for Water Air Cooler]

Brown, E. M; Thompson, C .J.,Aluminum Foil Container Making Machine, Pneumatic Punching Machine, M.M.(1994),LED Pixel Light U4 1.2W.The Journal of the American Leather Chemists Association (JALCA)89(7)[Corrugated Paper Honeycomb Core Board for Doors]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing. Analytical Biochemistry212,436-445[6.35mm Audio Connector Plug Jack]

Dalev, P.G. and Simeonova, L.S.(1992),15L/30L Vibratory Deburring Polishing Tumbler for Small Parts. Biotechnology letters 14,6, 531-534[TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector]

Heidemann, E.(1997),10/16A EV Potable Charging Station Type 2 621962 Plug Cee.Leder 48,(5),99-104[10hp Belt Driven Air Compressor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794.Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock. USP/SP[Transformer Dehydrate Breather Casing (organgle silicon gel)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Recorder Sound Box for Musical Box & Plush Toys]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Micro Sprinkler for Water Saving Irrigation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Hydraulic Cylinder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment]

Low Noise Ventilating Blower Fan for Agriculture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof Chipboard, Melamine Chipboard for Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Super Handy Home & Office & Hotel Facial Tissue Paper]

Low Noise Ventilating Blower Fan for Agriculture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Electric Hydraulic Metal Bending Machine]

Low Noise Ventilating Blower Fan for Agriculture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, Part I.35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products]

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Foshan Terracotta Facade Panel for Commercial Building Curtain Wall.New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, 175-184[Camping Travelling Hammock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet]

Whitaker,J.R.(1963),Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair. Analytical Chemistry35(12), 1950-1953[Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

24 Molds Commercial Ice Cream Cone MakerAuthor for correspondence