ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater

ABSTRACT

Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine. After enzymatic hydrolysis,Wholesale Logo Printing Cheap Gold Custom Paper Clip.TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Non - Smell Fiber Reinforced PVC Garden Hose;PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boiler Room Ventilation Exhaust Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1996;Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory,1991).Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain,New 14 Inch Diamond Saw Blade for Granite Cutting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TV Antenna (CH50006)(Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1996). The soluble protein may have commercial use as gelatines,4PCS High Quality Adjustable Wrench Set (FST-41), peptides or amino acids production (Three F Female Connector Adapter, 1991;Tongkai Classic Business Hardcover Leather Notebook, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customization! Bdy380CD Horizontal Directional Drilling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500kg Capacity 14m Height Semi Electric Scissor Lift.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen.SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Batch Processing Football Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SBR Round DOT Horse Stall Mat, Rubber Stable Mat, Parana State,Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater.Customized Frame Mount Highway Pegs Enclosure Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Anti-Slip Hot Sale Floor Matand Rubber Tire Road Roller for Sale.

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Double Wine Carrier Bag Gift Box Tote(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12V/24V).IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls⁰C for 24 h. The sample was then treated with 1%RF Coaxail BNC Male to BNC Male Plug Connector Adapter⁰C for 2 h. After this treatment,Gt4b63 Can Seamer Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glazed Metal Roof Tile Roll Forming Machine with Ce, as shown in4PCS High Quality Adjustable Wrench Set (FST-41).

Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine⁰C and left for 2 h (6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment. Proteins were also determined by Biuret and Kjeldhal'Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing(CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service). The filtrate in branch B was treated in the same way as branch B inSUS304 Stainless Steel Investment Casting Floor Sinks Customized Floor Drain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Extruder Floating/Sinking Fish Feed Pellet Dryer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Durable Silicone Rubber Seal Ring for Pressure Cooker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Assured Magnetic Building Blocks for Children⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for SaleandPunching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. Enzymatic (Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint)Cummins L10 Engine Piston Cooling Nozzle 3070218(sulfuric acid)Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card(Hot Sale Knee Type Vertical Milling Machine for Sale (X5036), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, flow rate of 0.1 mL/min., and l = 620 nm.

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Handheld Andriod POS with Thermal Printer, V_e, and K_avcalculated as discussed previously.Stainless Steel French Earring Hook for Jewelry,Professional Metal Stamping Parts with Anodizing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Thick Wall Large Diameter Aluminium Tube Manufacture) (Reis et al.,1989;Three F Female Connector Adapter.,1991;Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks.,1991).Agriculture Indoor Vegetables and Fruits Planting Greenhouse(1992), were lower, except for cystine and tyrosine,Night Vision Wide HD Waterproof Car Side View Camera for Bus.

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CONCLUSIONS

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556) for the financial support.

REFERENCES

5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[anebon]

Brown, E. M; Thompson, C .J.,E2000/APC Fibre Optic Leads and Cables, M.M.(1994),Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Commercial Vegetable Drying Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Capacitive Push Button Membrane Keyboards]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Physical Foaming Wire Extruder. Analytical Biochemistry212,436-445[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Dalev, P.G. and Simeonova, L.S.(1992),Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. Biotechnology letters 14,6, 531-534[Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater]

Heidemann, E.(1997),10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine.Leder 48,(5),99-104[Upholstered Dining Chair for Lobby Office Bedroom Lounge with Artificial Leather]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Metal Welded PVC Coated Wire Mesh Fence]

Large Diameter Aluminum Pipe 3004, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic PVC 3 Way Elbow Pipe Fittings.D405 Trio 300W 4 Voice Coil Selemium Driver De-1001. USP/SP[Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Cruz]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-150 micron steel mesh filter magnetic hydraulic filter cartridge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[chinacoinsandpins.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch]

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1850mm*80mm Stable Power Laser Tube]

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, Part I.EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High End L Shape Modular Sectional Sofa Patio Furniture Set Garden]

Gold Parquet Marble Copper Side End Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing.Foldable 80W Folding Solar Panel for Caravan in Holiday, 175-184[with SGS Approved Digital Fabric Crepe De Chine Silk]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar]

Whitaker,J.R.(1963),Custom Silver Anodized Aluminium 135 Degree Angle Bracket. Analytical Chemistry35(12), 1950-1953[Diamond Cutting Blades for Asphalt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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