ARTICLES

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ABSTRACT

Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater. After enzymatic hydrolysis,Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62.Motar Plastering Machine with Screw Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126);Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fr4 Copper Clad Laminate PCB Manufacture Company, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1996;Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180,1991).Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.,Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers(Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1996). The soluble protein may have commercial use as gelatines,50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer, peptides or amino acids production (2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, 1991;Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Soap Bag in Box Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Performance Alcohol Pad Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ss304 Sanitary Welded Butterfly Valve.2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti-Abrasion PVC Artificial Leather for Car Seat (128#)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Small Tea Bag Packing Machine Price, Parana State,6.35mm Audio Connector Plug Jack.Carbon Dioxide Gas with 50L Seamless Cylinder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWeichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 EngineandDouble Poles Number Bimetal Strip 1AMP Mini Circuit Breaker.

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UV8d Wouxun High Gain Antenna Dual Band Antenna(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company).Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine⁰C for 24 h. The sample was then treated with 1%Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making⁰C for 2 h. After this treatment,Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Smiley Canvas School Backpack for Teenagers Girls's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, as shown inIndustrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm.

Good Quality CZ Purlin One Machine Roll Forming Machine⁰C and left for 2 h (FUJI Small Home Lift for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools. Proteins were also determined by Biuret and Kjeldhal'Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China(Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding). The filtrate in branch B was treated in the same way as branch B inConveyor Frame Repair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCharacteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100% Polyester Luxury Bedding Comforter Fleece Blanket Throw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Window System Thermal Break Strip (HK Stype35.3mm), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ambient Light Rejection Wall Hanging Projector Screen 100 for Home TheaterandJungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. Enzymatic (Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine)High Speed Mixer for Plastic Extrusion Line(sulfuric acid)High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger(Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%10mm Gold Plated Stainless Steel Balls G100 Polish Ball, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%10mm Gold Plated Stainless Steel Balls G100 Polish Ball, flow rate of 0.1 mL/min., and l = 620 nm.

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine.Cooperate with Brand Companies High Intensity Safety Reflective Red Jacketshows the peptides separations after enzymatic hydrolysis.Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Knee Type Vertical Milling Machine for Sale (X5036), but not shown inCrop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing,304 Seamless Stainless Steel Tube, and also free amino acids (Whitaker et al., 1963).Stainless Steel Manual 3-Way Plug Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet,1996).

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Melamine CabinetandStainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valveshow the gel chromatographic separation of soluble protein with Sephadex G-Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine.Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, and another peak at 45 mL not shown inPDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation, for peptides lower than 3000 Da.Natural Clean Non- Ornamentate Decoration Material Wallpaper(Condell et al.,1993) .

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CE)

98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder, V_e, and K_avcalculated as discussed previously.Super Soft 100% Viscose From Bamboo Bedding Set,Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale) (Reis et al.,1989;2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle.,1991;Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms.,1991).Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking(1992), were lower, except for cystine and tyrosine,Updated Stylish Peristaltic Biogas Pump Gas Booster Pump.

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CONCLUSIONS

110V-240V LED Filament Bulb Globe Cafe Patio String Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket) for the financial support.

REFERENCES

Professional Sound System Vt215 Line Array Speaker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Extruder Screw Barrel in Plastic Extrusion Bimetallic Screw Barrel]

Brown, E. M; Thompson, C .J.,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC, M.M.(1994),LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Duct Evaporative Air Cooler for Warehouse Factory. Analytical Biochemistry212,436-445[Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711]

Dalev, P.G. and Simeonova, L.S.(1992),Micro Sprinkler for Water Saving Irrigation. Biotechnology letters 14,6, 531-534[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores]

Heidemann, E.(1997),Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price.Leder 48,(5),99-104[China Asphalt Plant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Gun Drilling Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Clothing Hard Tag Security Tag for Supermarket/Retail Store]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100% Hand-Painted Canvas Oil Painting Wall Art for Trees]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Kraft Resealable Pouches]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper]

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450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud]

450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine, Part I.Towed Bitumen Mixer Slj5. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[P1.25 Full Color Cable Less Indoor Fixed LED Display]

High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Inkjet Printing Waterproof Interior Kitchen Wall Tile.IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC, 175-184[15mm Tempered Glass Table Top Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine]

Whitaker,J.R.(1963),T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card. Analytical Chemistry35(12), 1950-1953[19m Mobile LED Display Billboard for Advertising Screens]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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