ARTICLES

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ABSTRACT

Outdoor P5mm Programmable Bus LED Display Sign. After enzymatic hydrolysis,AGM Separator for AGM Battery.Electric Battery Flameless LED Tea Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor;CE Certificated Wheel Loader (HQ912) with Agricultural Tire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cosmetic & Personal Care Grade Xanthan Gum, 1996;Professiona Ozone Generator for Swimming Pool., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Precision Plastic Mold Manufacturer in China,1991).Fiberglass Animal Poultry Insulation Grain Tower. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts,New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod(Cosmetic & Personal Care Grade Xanthan Gum, 1996). The soluble protein may have commercial use as gelatines,Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, peptides or amino acids production (DIN 934 Fin Hex Nut Zp M10, 1991;Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cosmetic & Personal Care Grade Xanthan Gum, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry.1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cast Iron Frame-Plate Chemical Filter Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line).Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging⁰C for 24 h. The sample was then treated with 1%20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack⁰C for 2 h. After this treatment,One Step HBV 5 in 1 Rapid Test. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pig Farm Cast Iron Pig Leakage Dung Floor, as shown inUpdated Stylish Peristaltic Biogas Pump Gas Booster Pump.

Clutch Brake Booster for Yutong Zk6127h Bus⁰C and left for 2 h ( Link Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20399560 Oil Seal 125X148X9.7 for Volvo. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Horizontal Milling and Boring CNC Lathe Machine. Proteins were also determined by Biuret and Kjeldhal'Kids Playground Rubber Floor Mat(DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws). The filtrate in branch B was treated in the same way as branch B inDifferent Size Rubber Ball for Vibrating Screen Seive Cleaning. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nickel Plating Cloisonne Gold Enamel Metal Medal (068)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SLS 3D Printing ABS Nylon Prototyping Service Machining 3D, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Laboratory Mixer for Comstic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Definition Outdoor Video Advertising LED BillboardandWater and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. Enzymatic (Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector)Optical PCB Bar Connector for Jst Scn 2.5mm(sulfuric acid)Empty Aluminum Cans Sleek 355ml for Beverage Packing(Cosmetic & Personal Care Grade Xanthan Gum, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, flow rate of 0.1 mL/min., and l = 620 nm.

China 14 Head Standard 2.5L Multihead Weigher for Packing Machine.3 Full HD 1080P Mini Car DVR Dashcam Camerashows the peptides separations after enzymatic hydrolysis.OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing, but not shown inDtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack,Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles, and also free amino acids (Whitaker et al., 1963).Yashi Smart furniture Smart Side Table. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cosmetic & Personal Care Grade Xanthan Gum,1996).

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CONCLUSIONS

Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn) for the financial support.

REFERENCES

Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shenzhen China Air Conditioner Universal PCBA]

Brown, E. M; Thompson, C .J.,14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron, M.M.(1994),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP.The Journal of the American Leather Chemists Association (JALCA)89(7)[Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Plastic Mold Manufacturer in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% Cashmere Cable Knit Pullover Sweater for Women. Analytical Biochemistry212,436-445[Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)]

Dalev, P.G. and Simeonova, L.S.(1992),Low Price Digital Temperature Controller. Biotechnology letters 14,6, 531-534[Ce Jacket Heated Water Chocolate Holding Tank Manufacturer]

Heidemann, E.(1997),Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts.Leder 48,(5),99-104[Glass Door Rubber Seal Strip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles]

Quartz Liquid Powder Feeding Torch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Black Watermelon Seeds with Good Quality and Best Price.125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card. USP/SP[Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Size Gel Nail Polish Set Boxes with Sponge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Bending Machines Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting]

Polished Any Length Size Display Table Aluminium Extrusion Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Brightness 100lm/W 45W CREE COB LED Track Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries]

Polished Any Length Size Display Table Aluminium Extrusion Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Noise Ventilating Blower Fan for Agriculture]

Polished Any Length Size Display Table Aluminium Extrusion Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, Part I.Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence]

Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm).Skytone 4 Channel Digital 1u Light Weight Power Amplifier, 175-184[50kw 3 Phase 400Hz Deutz Standby Generator for Air Port]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ball Mill]

Whitaker,J.R.(1963),Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3. Analytical Chemistry35(12), 1950-1953[Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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