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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Steel Rubber Seal for Dust Proof. After enzymatic hydrolysis,Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf.Kindergarten Playground Preschool Kids Plastic Slide and Swing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, 1996;Capacitive Push Button Membrane Keyboards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow,1991).High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet,Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper(New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, 1996). The soluble protein may have commercial use as gelatines,12V 96LED Outdoor Lighting Solar Torch Light for Christmas, peptides or amino acids production (Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line, 1991;SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Swimming Pool Air Source Water Heat Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box.QJ-M Moving small manual brick making machine with low cost-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic Waste Paper Stripper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (AGM Separator for AGM Battery).Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised⁰C for 24 h. The sample was then treated with 1%Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1⁰C for 2 h. After this treatment,U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)99% Purity Caustic Soda Flakes From Direct Manufacture on Sale, as shown inNew Design Steel Prefab Layer Cage Chicken House Poultry Farm.

Bwb-306 Custom Printing Outdoor Sports Wristband⁰C and left for 2 h (110V-240V LED Filament Bulb Globe Cafe Patio String Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Irregular Shape Hologram Label Printing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Speed Rope Weaving Machine. Proteins were also determined by Biuret and Kjeldhal'Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill(Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay). The filtrate in branch B was treated in the same way as branch B inPE Film Bag Sealing and Cutting Power Line Packaging Machine Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFunny Kids Soft Play Maze Big Slide Indoor Soft Playground(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Durable 25kg 50kg Red PP Woven Bag Sack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mini Recorder Sound Box for Musical Box & Plush Toys, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Precision iPhone Cases Fiber Laser Marking MachineandFake Fish Luminescent Tropical Fish Fish Tank Decoration. Enzymatic (Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine)High Pressure Die Casting Aluminum Die Casting Gear Box Housing(sulfuric acid)Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator(New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVC Hdo Construction Formworks) for the financial support.

REFERENCES

Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[56105-45V27 Auto Suspension Parts Front Right Japanese Shock Absorber for Nissan]

Brown, E. M; Thompson, C .J.,New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve, M.M.(1994),High Quality RV Electric Seating Recliner.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Floral Kids PVC School Raincoat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc. Analytical Biochemistry212,436-445[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Dalev, P.G. and Simeonova, L.S.(1992),100% Hand-Painted Canvas Oil Painting Wall Art for Trees. Biotechnology letters 14,6, 531-534[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Heidemann, E.(1997),Factort Price Cattle Beef Slaughtering Equipment for Sale.Leder 48,(5),99-104[China Custom Swing Tags String Hang Tag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Model Water Source Heat Pump (SLW100D)]

High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Supreme 524 Mv Sea Kayak.API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve. USP/SP[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manual Glass Lifting Machine for Making Double Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stackable Nestable Plastic Box Container with Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

High Definition Outdoor Video Advertising LED Billboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

High Definition Outdoor Video Advertising LED Billboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Community IC Access Card Induction ID Time Card Smart Card]

High Definition Outdoor Video Advertising LED Billboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:304 Stainless Steel Expansion Anchor Bolt, Part I.New Small Plug-in Insect Killer Mosquito Killing Lamp. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Waste Paper Stripper]

Wholesale Customize PU Leather Hardcover Notebook with Window, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11).Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper, 175-184[China Die Casting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

Whitaker,J.R.(1963),33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer. Analytical Chemistry35(12), 1950-1953[Extruder Floating/Sinking Fish Feed Pellet Dryer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production LineAuthor for correspondence