ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Soap Bag in Box Packing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting

ABSTRACT

High Performance Alcohol Pad Making Machine. After enzymatic hydrolysis,Ss304 Sanitary Welded Butterfly Valve.2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Anti-Abrasion PVC Artificial Leather for Car Seat (128#);Digital Control with LED Display DC Solar Small Deep Freezer Fridge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Economical Check Weigher with High Speed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, 1996;Steel Handle Rake Flat Rake Garden Rake Agricultural Rake., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Small Tea Bag Packing Machine Price,1991).Carbon Dioxide Gas with 50L Seamless Cylinder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UV8d Wouxun High Gain Antenna Dual Band Antenna,Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine(Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, 1996). The soluble protein may have commercial use as gelatines,Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, peptides or amino acids production (Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift, 1991;Smiley Canvas School Backpack for Teenagers Girls, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality CZ Purlin One Machine Roll Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools.Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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8 Acetic Acid Sds

Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet).Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Mixer for Plastic Extrusion Line⁰C for 24 h. The sample was then treated with 1%High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger⁰C for 2 h. After this treatment,10mm Gold Plated Stainless Steel Balls G100 Polish Ball. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags, as shown inAutomatic Hexagonal Wire Netting Machine Gabion Mesh Knitting.

304 Seamless Stainless Steel Tube⁰C and left for 2 h (42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Manual 3-Way Plug Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine. Proteins were also determined by Biuret and Kjeldhal'Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck(New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic). The filtrate in branch B was treated in the same way as branch B inTruck Paint Booth for Construction Equipment Paint Spray Booth Portable Industrial Paint Booth. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Natural Clean Non- Ornamentate Decoration Material Wallpaper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuper Soft 100% Viscose From Bamboo Bedding Set(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/110V-240V LED Filament Bulb Globe Cafe Patio String Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price.PVC Car Seat Belt Shoulder Protector 80438shows the peptides separations after enzymatic hydrolysis.High End L Shape Modular Sectional Sofa Patio Furniture Set Garden(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Anti-Slip Hot Sale Floor Mat, but not shown in Rubber Tire Road Roller for Sale,ABS Shell Plastic Injection Molding, and also free amino acids (Whitaker et al., 1963).Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket,1996).

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Towed Bitumen Mixer Slj5, V_e, and K_avcalculated as discussed previously.High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price,3D Inkjet Printing Waterproof Interior Kitchen Wall Tile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale) (Reis et al.,1989;Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift.,1991;Steel Handle Rake Flat Rake Garden Rake Agricultural Rake.,1991).IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC(1992), were lower, except for cystine and tyrosine,T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card.

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CONCLUSIONS

Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Container House / Modular Residential / Prefabricated Mobile Home) for the financial support.

REFERENCES

Viscose Sodium Sulphate Anhydrous by Sateri, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3kg Enzyme Laundry Washing Powder / Detergent Powder]

Brown, E. M; Thompson, C .J.,Orange Silica Gel 2-4 mm with High Quality, M.M.(1994),2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pillow Case Automatic Sewing Machine Bc901]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-aluminium discs for fry pan alloy 1060 1100 1050 3003. Analytical Biochemistry212,436-445[High Pressure Low Volume 2900rpm electric Water Pump]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Insulated Double Wall Bamboo Glass Water Bottle. Biotechnology letters 14,6, 531-534[Fire Retardant Reflective Aluminium Foil Woven for Insulation Material]

Heidemann, E.(1997),Aroma Reed Diffuser with Ratten Sticks for Home Fragrance.Leder 48,(5),99-104[LED High Bay Light 150W with Reflector Prismatic Diffusers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cosmetic & Personal Care Grade Xanthan Gum]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Thick Wall Large Diameter Aluminium Tube Manufacture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sw-Se-006 2019 New Product Luxury Interior Solid Wood Timber Door]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing]

16oz Square Shape Colors Glass Mason Jar with Embossed Design, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Vegetable Drying Equipment]

16oz Square Shape Colors Glass Mason Jar with Embossed Design,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Capacitive Push Button Membrane Keyboards]

16oz Square Shape Colors Glass Mason Jar with Embossed Design, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, Part I.100ml Plastic E Liquid Vape Cig Juice Dropper Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags.2-25mm Co-Extruded Highlight PVC Foam Board, 175-184[Summer Silk Pajamas]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater]

Whitaker,J.R.(1963),CNC Engraving Machine Laser Marking for Assembly Line. Analytical Chemistry35(12), 1950-1953[Galvanized Metal Welded PVC Coated Wire Mesh Fence]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly ValveAuthor for correspondence