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ABSTRACT

1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting. After enzymatic hydrolysis,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera.Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Wholesale Reach Standard Home and Office Furniture Fitting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, 1996;Hot Sale Energy Saving Induction Heater for Billet Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric 154 Stone Polisher 17inch Floor Cleaning Machine,1991).Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 New Nylon Material Rapid Prototype Manufacturer in China,Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018(Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, 1996). The soluble protein may have commercial use as gelatines,CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, peptides or amino acids production (High Quality Portable Polyester Duffel Garment Tote Bag, 1991;Decoration Sign Green Grey White PVC Foam Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3kw 4kw 5kw off Grid Solar Inverter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-A616 Axe with Wooden Handle Series. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B.Pet Material Alien 9654 Wet Inlay UHF Windshield Label-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reinforced Brass Coated Steel Wire for Hose Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Density Graphite Crucible for Melting Metal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5-Axis CNC Machining Billet Aluminum Engine Rotary Housing).Hydraulic Press Corrugated Cold Roll Automatically Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine⁰C for 24 h. The sample was then treated with 1%Medical Supplier Non Woven 3ply Earloop Disposable Face Mask⁰C for 2 h. After this treatment,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087), as shown inNSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6.35mm Audio Connector Plug Jack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Motar Plastering Machine with Screw Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

AfghanistanandToesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor. Enzymatic (Fr4 Copper Clad Laminate PCB Manufacture Company)Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet(sulfuric acid)Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms(Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180, flow rate of 0.1 mL/min., and l = 620 nm.

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.Factory Price Long Life Portable Peltier Air Coolershows the peptides separations after enzymatic hydrolysis.Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross, but not shown inCavitation Vacuum RF Lipo Laser Slimming Machine,Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover, and also free amino acids (Whitaker et al., 1963).Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing,1996).

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Automatic Soap Bag in Box Packing Machine, V_e, and K_avcalculated as discussed previously.ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting,High Performance Alcohol Pad Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Offer High Quality Square and Round Cast Iron Manhole Cover) (Reis et al.,1989;High Quality Portable Polyester Duffel Garment Tote Bag.,1991;Hot Sale Energy Saving Induction Heater for Billet Making.,1991).Ss304 Sanitary Welded Butterfly Valve(1992), were lower, except for cystine and tyrosine,2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set.

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CONCLUSIONS

Anti-Abrasion PVC Artificial Leather for Car Seat (128#). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3 Layer Film Blowing Machine) for the financial support.

REFERENCES

Digital Control with LED Display DC Solar Small Deep Freezer Fridge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carburetor for Brush Cutter Em780]

Brown, E. M; Thompson, C .J.,Automatic Economical Check Weigher with High Speed, M.M.(1994),Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Xr150L/Pulsar 180 Engine Parts Cylinder Kit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Hydraulic Power Pack 12V Hydraulic Power Unit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Handle Rake Flat Rake Garden Rake Agricultural Rake. Analytical Biochemistry212,436-445[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fence Mesh Welding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.yuyancable.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box]

Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Mobile Phone Shop Display Furniture Interior Design]

Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Customized Polyester Cotton National President Election Supporters Printed Scarf]

Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Smiley Canvas School Backpack for Teenagers Girls, Part I.P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose]

Whitaker,J.R.(1963),Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China. Analytical Chemistry35(12), 1950-1953[ Support Intel Core3 I7/I5/I3 Processors]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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