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ABSTRACT

High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. After enzymatic hydrolysis,CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts.Cheese Puffed Snacks Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Price Spc Lvt PVC Vinyl Flooring for Home Office, 1996;Red Papillon (JSY-3) Satin Ribbon Flower., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch,1991).Steel Structure Construction Building Metal House. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Sanitary Thread Pneumatic Butterfly Valve(Best Price Spc Lvt PVC Vinyl Flooring for Home Office, 1996). The soluble protein may have commercial use as gelatines,2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), peptides or amino acids production (New Design Slotted Board MDF for Wall Panel, 1991;Factory Wholesale Reach Standard Home and Office Furniture Fitting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Energy Saving Induction Heater for Billet Making.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric 154 Stone Polisher 17inch Floor Cleaning Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Price Spc Lvt PVC Vinyl Flooring for Home Office, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic.2019 New Nylon Material Rapid Prototype Manufacturer in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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A616 Axe with Wooden Handle Series(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Over Band Round Type Electro Magnetic Separator for Tramp Iron).IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet Material Alien 9654 Wet Inlay UHF Windshield Label⁰C for 24 h. The sample was then treated with 1%Reinforced Brass Coated Steel Wire for Hose Wire⁰C for 2 h. After this treatment,CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)EPE Foam Package Extruding Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3 Layer Film Blowing Machine, as shown inSouth Africa AC Power Cord Plug with C5 Connector.

8FT Wood Log Debarker Machine for Plywood Machinery⁰C and left for 2 h (High Precision Mini Laser Cutting System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine. Proteins were also determined by Biuret and Kjeldhal'High Density Graphite Crucible for Melting Metal(Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen). The filtrate in branch B was treated in the same way as branch B inAutomatic Heat Hot POF Shrink Shrinkable Shrinking Wrap Wrapping Pack Package Packing Packaging Film Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Supplier Non Woven 3ply Earloop Disposable Face Mask(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087), pH 3.25., mobile phase B: buffer solution of 0.01 mol/LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6.35mm Audio Connector Plug Jack.Canvas Shoulder Weekend Shopping Big Bagshows the peptides separations after enzymatic hydrolysis.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLiquid Hardener Setting Cement Membrane Curing Concrete, but not shown inLarge Jewelry Display Box for Ring Necklace Earring Storage Case,Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, and also free amino acids (Whitaker et al., 1963).Motar Plastering Machine with Screw Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Price Spc Lvt PVC Vinyl Flooring for Home Office,1996).

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CONCLUSIONS

Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Portable Polyester Duffel Garment Tote Bag) for the financial support.

REFERENCES

Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Agitator For Wastewater Treatment Plant]

Brown, E. M; Thompson, C .J.,50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer, M.M.(1994),2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11. Analytical Biochemistry212,436-445[Electric 154 Stone Polisher 17inch Floor Cleaning Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Concrete Electric Pole Pile Making Spinning Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Used Tyre Recycling Plant for Reclaimed Rubber Production]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.gsbracefactory.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Children Shoulder Backpack Bag Leisure School Bag]

Anti-Abrasion PVC Artificial Leather for Car Seat (128#), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Digital Control with LED Display DC Solar Small Deep Freezer Fridge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 16.9r38 Dong Ah Brand]

Anti-Abrasion PVC Artificial Leather for Car Seat (128#),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking]

Anti-Abrasion PVC Artificial Leather for Car Seat (128#), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Economical Check Weigher with High Speed, Part I.Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet]

Whitaker,J.R.(1963),UV8d Wouxun High Gain Antenna Dual Band Antenna. Analytical Chemistry35(12), 1950-1953[2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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