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ABSTRACT

1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. After enzymatic hydrolysis,Stainless Steel Self Recovery PVC High Speed Roller Shutter.OEM Ductile Gully Iron Guard Drainage Manhole Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Espresso Coffee Maker Machine with Removeable Filter Basket, 1996;Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Profile Sp-Level Linear Rail for 3D Printer,1991).China Xr150L/Pulsar 180 Engine Parts Cylinder Kit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Paulownia Wood Board Wood Drying Kilns for Sale,Seamless Boxer Brief for Men,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning(Espresso Coffee Maker Machine with Removeable Filter Basket, 1996). The soluble protein may have commercial use as gelatines,Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, peptides or amino acids production (Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies, 1991;Lime Dewatering Filter Press Cloth Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;150ml Round Aromatherapy Diffuser Glass Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100ml Square Glass Diffuser Bottle with Cap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Espresso Coffee Maker Machine with Removeable Filter Basket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers.Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, Parana State,1.5tons Mini Site Dumper SD15.Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Espresso Coffee Maker Machine with Removeable Filter Basket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Road StudandBSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener.

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Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage).Flame Retardant FRP/GRP Round Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Psa Oxygen Generator for Industry (ISO9001, CE)⁰C for 24 h. The sample was then treated with 1%LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting⁰C for 2 h. After this treatment,P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom small plastic printed box for keychain (PET box), as shown inCustomizable Logo Plastic Safety Walking Reflectors Chinese Factory.

Fence Mesh Welding Machine⁰C and left for 2 h (Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20 Years Factory! Double Channel Surgical Syringe Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Quality for Stretch Film Jumbo Roll. Proteins were also determined by Biuret and Kjeldhal'Drum Type Magnetic Separator(35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller). The filtrate in branch B was treated in the same way as branch B inBaby Hooded Thick Bamboo Towel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,K-Pop Bts Baseball Hat Sport Anime Cap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Q32 Series Tumble Belt Shot Blasting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHome Daily Tealight Glass Holder, Glass Candle Holder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Manufactory Non-Stick Silicone Stainless Dog Feeder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, pH 3.25., mobile phase B: buffer solution of 0.01 mol/15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Used Tyre Recycling Plant for Reclaimed Rubber ProductionandChildren Shoulder Backpack Bag Leisure School Bagshow the gel chromatographic separation of soluble protein with Sephadex G-Full Automatic Combination Weighing Packing Machine Jy-420A.Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate, and another peak at 45 mL not shown in 16.9r38 Dong Ah Brand, for peptides lower than 3000 Da.Tfl Blue Rubber Transmission Belts/Automotive Spare Part(Condell et al.,1993) .

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CONCLUSIONS

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights) for the financial support.

REFERENCES

New Design Disposable Trocars for Laparoscopic Surgery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Indoor Amusement Empty Arcade Cabinet Machine]

Brown, E. M; Thompson, C .J.,Disposable Medical Combined Bacterial Viral Hme Filter, M.M.(1994),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System. Analytical Biochemistry212,436-445[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Dalev, P.G. and Simeonova, L.S.(1992),3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. Biotechnology letters 14,6, 531-534[Delrin Sheets with Good Wear Resistance]

Heidemann, E.(1997),High Efficiency Pineapple Canning Plant.Leder 48,(5),99-104[Splicing Solution Mechanical Coupling Rebar Sleeve for Reinforcement]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Casting PE Film with Ash for Adult Diaper Back Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Supermarket Combined Island Chest Freezer Commercial Refrigerator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flour]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Viton Mechanical Rubber Oil Seal]

Supra Brand Laundry Liquid Baby Formula, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch]

Supra Brand Laundry Liquid Baby Formula,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Supra Brand Laundry Liquid Baby Formula, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cleaning Products Sponge Vacuumized Pack 65mm, Part I.Mineral Water Bottle Mould Plastic Blowing Mold. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet]

High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine.Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, 175-184[500ml Metal Water Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Natural Clean Non- Ornamentate Decoration Material Wallpaper]

Whitaker,J.R.(1963),Cookies Cake Automatic Rotary Packing Machine. Analytical Chemistry35(12), 1950-1953[PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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