ARTICLES

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ABSTRACT

10mm Gold Plated Stainless Steel Balls G100 Polish Ball. After enzymatic hydrolysis,Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine.Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;304 Seamless Stainless Steel Tube;Stainless Steel Manual 3-Way Plug Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, 1996;Natural Clean Non- Ornamentate Decoration Material Wallpaper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder,1991).Super Soft 100% Viscose From Bamboo Bedding Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Theme Jungle Plastic Stainless Steel Slide Outdoor Playground,Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Updated Stylish Peristaltic Biogas Pump Gas Booster Pump(Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, 1996). The soluble protein may have commercial use as gelatines,110V-240V LED Filament Bulb Globe Cafe Patio String Light, peptides or amino acids production (Professional Sound System Vt215 Line Array Speaker, 1991;Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Duct Evaporative Air Cooler for Warehouse Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Micro Sprinkler for Water Saving Irrigation. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price.High End L Shape Modular Sectional Sofa Patio Furniture Set Garden-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ABS Shell Plastic Injection Molding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D Inkjet Printing Waterproof Interior Kitchen Wall Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag).IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card⁰C for 24 h. The sample was then treated with 1%Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick⁰C for 2 h. After this treatment,Viscose Sodium Sulphate Anhydrous by Sateri. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Orange Silica Gel 2-4 mm with High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI, as shown inMini Succulent Planters.

aluminium discs for fry pan alloy 1060 1100 1050 3003⁰C and left for 2 h (Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Insulated Double Wall Bamboo Glass Water Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aroma Reed Diffuser with Ratten Sticks for Home Fragrance. Proteins were also determined by Biuret and Kjeldhal'High Quality Fashion Ladies Backpack PU Leather Backpack(Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine). The filtrate in branch B was treated in the same way as branch B inPrinter Scanner POS and EMV for Payment Store and Kiosk PT7003. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Suction Filter Cylinder Match 48 Series Filter Brands.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av16oz Square Shape Colors Glass Mason Jar with Embossed Design(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100ml Plastic E Liquid Vape Cig Juice Dropper Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt FabricandPolypropylene Woven 25kg 50kg Bags Wholesale Sand Bags. Enzymatic (2-25mm Co-Extruded Highlight PVC Foam Board)CNC Engraving Machine Laser Marking for Assembly Line(sulfuric acid)PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)(Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manual Glass Lifting Machine for Making Double Glass) for the financial support.

REFERENCES

Liquid Hardener Setting Cement Membrane Curing Concrete, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine]

Brown, E. M; Thompson, C .J.,Professional Silicone Rubber Gasket for Doors Windows, M.M.(1994),Top Quality 99.95% Molybdenum Square Plates.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2-25mm Co-Extruded Highlight PVC Foam Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee. Analytical Biochemistry212,436-445[Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box]

Dalev, P.G. and Simeonova, L.S.(1992),Flat Face Stainless Steel Female Jic Quick Release Coupling. Biotechnology letters 14,6, 531-534[Household Use Food Packing 8011 Aluminum Foil]

Heidemann, E.(1997),Aluminium Glass Dropper for Glass Dropper Bottle.Leder 48,(5),99-104[Theodoor Air Source Heat Pump PV Function for Solar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition]

Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),L133pbd Common Rail Delphi Nozzle for Ford Mondeo.Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing. USP/SP[3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dubai Products Rectified Tile for Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[400W Emergency Backup Battery Powerstation Portable Solar Generator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Swage Stud Thrdaded Terminal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Car Interior Accessories Customized Car Seat Cover]

Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),One Compartment Commercial Sink Without Drainboard-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men]

Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Professional Design Wing Shape Engraved Car Label Emblem]

Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ge Holter Cable Snap Type Holter ECG Cable, Part I.Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh]

PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),65 Polyester 35 Cotton Fabric Camouflage Printed Fabric.Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, 175-184[Vodafast DIN7505 Cross Recessed Countersunk Head Chipboard Screw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask]

Whitaker,J.R.(1963),Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump. Analytical Chemistry35(12), 1950-1953[ Luxury Rainfalls Shower Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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