ARTICLES

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ABSTRACT

Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. After enzymatic hydrolysis,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection.3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;5-Axis CNC Machining Billet Aluminum Engine Rotary Housing;EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Clamshell Book Shape Black Cardboard Magnet Gift Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashionable Simple Printing Mini Key & Coin Purse, 1996;CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kids Folding Play Mat Kids Play Room Floor Mat,1991).Roller Window Shutter for Residential Usage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pre-Seasoned Cast Iron 5-Piece Cookware Set,Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel(Fashionable Simple Printing Mini Key & Coin Purse, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Hose SAE 100 R1AT, peptides or amino acids production (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, 1991;L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ts-Micro Portable Defense Loudspeaker System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashionable Simple Printing Mini Key & Coin Purse, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Supply for Laparoscopic Instrument (MIM Straight Scissors).86bl3a125 1.65A 660W 310V BLDC-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tamper Evident Branded Printed Packing Pet Adhesive Security Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Price of Peanut Digger / Groundnut Harvester in Australian Africa, Parana State,Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part.China Manufacture Table Type CNC Horizontal Boring Milling Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashionable Simple Printing Mini Key & Coin Purse, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-SweaterandGalvanized Metal Welded PVC Coated Wire Mesh Fence.

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White Electrical Fireplace Warmer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Cruz).Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod⁰C for 24 h. The sample was then treated with 1%4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone⁰C for 2 h. After this treatment,1 Inch Stainless Steel Flexible Exhaust Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, as shown inGuide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting.

Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator⁰C and left for 2 h (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ce Certificate High Security Steel Shackle Padlock. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Drive Shaft for Jmc 8-94134458-PT. Proteins were also determined by Biuret and Kjeldhal'DSG Ball Screw Fixed Seat Nut Support(Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch). The filtrate in branch B was treated in the same way as branch B in8inche Quality Pet Grooming Hair Cutter Dog Scissors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Apex Digital UV Printers for Aluminum Board Printing UV6090. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av24 Molds Commercial Ice Cream Cone Maker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Children Fur Pompom Beanie Scarf Knit Set⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel Jacket Steam Insulation Pipeline Production Best Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Haa 800mAh NiMH Battery Pack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber.High End L Shape Modular Sectional Sofa Patio Furniture Set Gardenshows the peptides separations after enzymatic hydrolysis.Custom Precision Aluminum Die Casting for Bicycle Accessories(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar, but not shown inDiamond Cutting Blades for Asphalt,China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25, and also free amino acids (Whitaker et al., 1963).Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashionable Simple Printing Mini Key & Coin Purse,1996).

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CONCLUSIONS

110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch) for the financial support.

REFERENCES

China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Abstract 2019 New Design (141M0071) Handmade Oil Painting Wall Decorative Art]

Brown, E. M; Thompson, C .J.,1300 Paper Slitting and Rewinding Machine, M.M.(1994),High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets.The Journal of the American Leather Chemists Association (JALCA)89(7)[K-Pop Bts Baseball Hat Sport Anime Cap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UL Certified 12V 277VAC Smart Relay for Utility Meters. Analytical Biochemistry212,436-445[Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3]

Dalev, P.G. and Simeonova, L.S.(1992),Over 95% Accessories Exported Custom Zipper in Rolls. Biotechnology letters 14,6, 531-534[E2000/APC Fibre Optic Leads and Cables]

Heidemann, E.(1997),Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory.Leder 48,(5),99-104[Carbon Steel Asme B36.10 Pipe Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China 100% PU Synthetic Leather for Shoes.HD Video Wall P3.91 LED Panel Display Indoor. USP/SP[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Reusable Silicone Food Storage Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Metal Stamping Stainless Steel Parts with CNC Machining: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Titanium Dioxide In Skin Care]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Epoxy Resins-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Vodka]

FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Nylon Sheet with High Temperature]

FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, Part I.24 Cores MPO/MTP Cassette. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Automatic Concrete Paver Interlocking Brick Block Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lightweight Design Industrial Robot with Six Axis for Painting.Small Diameter Coil Compression Spring From Factory, 175-184[Mommy Crystal Pregnant Toothpaste]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Lumen Outdoor IP65 100W LED Flood Light]

Whitaker,J.R.(1963),FDA Standard BBQ Skewer with Colorful Wooden handle. Analytical Chemistry35(12), 1950-1953[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple MouldAuthor for correspondence