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ABSTRACT

Nice Appearance Brass Metal Sheet Stamping. After enzymatic hydrolysis,Hot Sale Starter Motor Testing Equipment for Truck, Bus.Home Machine 2019 New Commercial Treadmill Gym Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic;Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Clothing Hard Tag Security Tag for Supermarket/Retail Store, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (150 Ton Hydraulic Press Machine for Cooking Pot Making, 1996;Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Flat New Design Ladies Winter Warm Shoes,1991).Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer,China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal(150 Ton Hydraulic Press Machine for Cooking Pot Making, 1996). The soluble protein may have commercial use as gelatines,IP67 High-Ending Industrial Socket / Connector, peptides or amino acids production (Mc4 Solar PV Connector TUV Approval, 1991;PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Over Band Round Type Electro Magnetic Separator for Tramp Iron.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (150 Ton Hydraulic Press Machine for Cooking Pot Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine.0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bgf-800 Pet Dry Laminating Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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HAIRUI 850A+ 600W SMD Rework Soldering Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat).High Quality Oval Shape Cast Iron Dutch Oven(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara⁰C for 24 h. The sample was then treated with 1%Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin⁰C for 2 h. After this treatment,Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, as shown inM16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6.

High Performance Fresh Air Handling Unit for Marine⁰C and left for 2 h (0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Community IC Access Card Induction ID Time Card Smart Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Food Packing Machine Biscuit Packing Machine with Auto Feeder. Proteins were also determined by Biuret and Kjeldhal'High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing(Full Automatic Combination Weighing Packing Machine Jy-420A). The filtrate in branch B was treated in the same way as branch B inChina Color Box Sealing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Military Uniform ISO and SGS Standard for Army Camouflage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%300W-2kw Solar Panel and Wind Turbine Hybrid System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/RV UPS Power 48V 80ah LiFePO4 Battery Pack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Popular Cheap Custom Logo Black Silicone Wristband in High Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Road Stud.New Virgin Teflon PTFE Teflon Gaskets/Back up Ringsshows the peptides separations after enzymatic hydrolysis.Lab Water Instruments/Lab Water Purification System Micro(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOne to One Order Following Strong Real Gold Thread, but not shown in Video Camera,Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia, and also free amino acids (Whitaker et al., 1963).Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (150 Ton Hydraulic Press Machine for Cooking Pot Making,1996).

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CONCLUSIONS

Pfc Inductors with Adjustable Dimension and Wire Diameter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating) for the financial support.

REFERENCES

Automatic Permanent Magnetic Lifter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Matt Pet Film /Matte Polyester Film for Food Flexible Packaging]

Brown, E. M; Thompson, C .J.,Large Diameter Aluminum Pipe 3004, M.M.(1994),Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Irregular Shape Hologram Label Printing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater. Analytical Biochemistry212,436-445[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine. Biotechnology letters 14,6, 531-534[High Quality Fashion Ladies Backpack PU Leather Backpack]

Heidemann, E.(1997),Wholesale Logo Printing Cheap Gold Custom Paper Clip.Leder 48,(5),99-104[Auto Espresso Coffee Vending Machine F305]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Dioxide Gas with 50L Seamless Cylinder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[24 Cores MPO/MTP Cassette]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Personalized Promotional Children Full Color Logo Printed T-Shirt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price]

Boiler Room Ventilation Exhaust Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill]

Boiler Room Ventilation Exhaust Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings]

Boiler Room Ventilation Exhaust Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, Part I.500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pre-Seasoned Cast Iron 5-Piece Cookware Set]

Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain.New 14 Inch Diamond Saw Blade for Granite Cutting, 175-184[m.sinoriseind.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles]

Whitaker,J.R.(1963),TV Antenna (CH50006). Analytical Chemistry35(12), 1950-1953[19gauge Galvanized Twin Binding Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CE Certificated Wheel Loader (HQ912) with Agricultural TireAuthor for correspondence