ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter

ABSTRACT

Quality Printed Cigarette Tipping Paper. After enzymatic hydrolysis,White Latex Paper Yellow/White Stearate Coated Abrasive Paper.Manufacturer Automotive CNC Machined Parts Checking Fixture Components. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting;Telescopic Slideway Steel Protection Accordion Cover; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, 1996;Ridge Cap Panel Forming Machine Ridge Cap Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Grinding Factory Corn Maize Flour Milling Mill Making Machines,1991).Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Construction & Decoration ERW Square Steel Tube Manufacturer,Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Household Use Food Packing 8011 Aluminum Foil(Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, 1996). The soluble protein may have commercial use as gelatines,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, peptides or amino acids production (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, 1991;Plastic Case Navigation System Tooling Injection Mold, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8X8mm Silver Expanded Metal Mesh in Hot Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100% Durable Flooring Roll for Hotel Office School and Health Project Floor.Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet Bottle Washing Recycling Line in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sc/APC Fiber Optic Fast Hot Melt Connector, Parana State,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHalogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)andDimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse.

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Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine).Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium⁰C for 24 h. The sample was then treated with 1%Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts⁰C for 2 h. After this treatment,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, as shown inHerrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts.

4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer⁰C and left for 2 h (Light Weight Ohsas 18001 Certified Metal Arch Buildings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Food Grade PE Coated Kraft Paper for Paper Cup. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Single Filter Cartridge Housing for Food Grade. Proteins were also determined by Biuret and Kjeldhal'Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration(High Performance Alcohol Pad Making Machine). The filtrate in branch B was treated in the same way as branch B inRubber Foaming Balls for Pipe Cleaning. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Stainless Steel Investment Casting Bearing for Car Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price of Custom Brands Granite Sink for Home⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/A36 Galvanized Building Steel Structure Column H Steel Column, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glass Board Automatic Sloping Printing Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event) for the financial support.

REFERENCES

Luxury Hotel Furniture Antique Design Marble Side Table, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hero Brand Flat Knitting High Table Cartonbox Box Semi Auto Carton Price Multifunctional Strapping Machine]

Brown, E. M; Thompson, C .J.,High Dust Holding Capacity HEPA Filter with Separator, M.M.(1994),Crystal Pendant Lamp, DIY Shade (WHP-455).The Journal of the American Leather Chemists Association (JALCA)89(7)[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1.5tons Mini Site Dumper SD15. Analytical Biochemistry212,436-445[Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box]

Dalev, P.G. and Simeonova, L.S.(1992),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. Biotechnology letters 14,6, 531-534[Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109]

Heidemann, E.(1997),Stainless Steel Self Recovery PVC High Speed Roller Shutter.Leder 48,(5),99-104[57 Dldc Motor 60W 3000rpm 24V Brushless DC Motor with Four Stage Rotor Design]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FTTH Optical Network Terminal for Gpon ONU and CATV]

Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Ductile Gully Iron Guard Drainage Manhole Cover.High Precision Rail Slider 20mm Diameter PMI Linear Guideway. USP/SP[Electric Automotive Heater Control Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Single Filter Cartridge Housing for Food Grade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Price Cat Scratching Trees Cat Tree Luxury: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor LED Decorative Street Linear Wall Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Banquet Spandex Chair Cover Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart]

China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Espresso Coffee Maker Machine with Removeable Filter Basket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine]

China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cookies Cake Automatic Rotary Packing Machine]

China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, Part I.Low Profile Sp-Level Linear Rail for 3D Printer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM/ODM Raw Material MDF HDF Laminate Door]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Paulownia Wood Board Wood Drying Kilns for Sale.Seamless Boxer Brief for Men, 175-184[Electric Paint Sprayer Spare Parts for Wagner 3.34]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PP PE PVC Single Wall Corrugated Pipe Making Machine Line]

Whitaker,J.R.(1963),Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. Analytical Chemistry35(12), 1950-1953[High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Stainless Steel Investment Casting Bearing for Car PartsAuthor for correspondence