ARTICLES

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ABSTRACT

OEM ODM Aluminum Casting Parts. After enzymatic hydrolysis,High Pressure Low Volume 2900rpm electric Water Pump.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240);Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multifunction Copper Busbar Processing for Aluminum Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1996;Electric DC 24V Geared Motor for Auto Doors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided,1991).RF Shielding MRI Door with Brass Frame for RF Cage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Geotextile Beg Used for Beach Restoration Geobag,Reusable Silicone Food Storage Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dental Emax Empress Crown From China Dental Lab(Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1996). The soluble protein may have commercial use as gelatines,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, peptides or amino acids production (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1991;Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dubai Products Rectified Tile for Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ANSI B16.5 Stainless Steel SS316 Forging Blind Flange.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer.Integrated Hydraulic Steering Control Units (BZZ1F SCU)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Model Water Source Heat Pump (SLW100D)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Capacity Vibrating Screen Manufacturer, Parana State,Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh.Electrochemical Polishing Tungsten Evaporation Materials, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLime Dewatering Filter Press Cloth FilterandSilica Gel- Indicating Blue to Pink After Adsorption.

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Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV).White UTP Cat5e UK Type Tooless RJ45 Keystone Jack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill⁰C for 24 h. The sample was then treated with 1%CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts⁰C for 2 h. After this treatment,Cheese Puffed Snacks Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China High Profit Automatic Pet Extruder Machine, as shown inDigital Floating Baby Care Products Frog Bath Design Thermometer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Slotted Board MDF for Wall Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Wholesale Reach Standard Home and Office Furniture Fitting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

EPE Foam Package Extruding Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets) for the financial support.

REFERENCES

3 Layer Film Blowing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Wipe Packaging Machine]

Brown, E. M; Thompson, C .J.,8FT Wood Log Debarker Machine for Plywood Machinery, M.M.(1994),Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Latex Paper Yellow/White Stearate Coated Abrasive Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine. Analytical Biochemistry212,436-445[High Speed Rope Weaving Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Density Graphite Crucible for Melting Metal. Biotechnology letters 14,6, 531-534[All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush]

Heidemann, E.(1997),Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.Leder 48,(5),99-104[Life P04 40b19L 12V24ah 450CCA Lithium Iron Phosphate Car Battery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nocle-125 Ce Certification Nc100le RCBO]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Bedsheet Folding Machine (ZD-3000)]

Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System]

Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml]

Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, Part I.Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[National Day Holiday Football Bravo Hand Weaving Flag]

New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape.19gauge Galvanized Twin Binding Wire, 175-184[3.2mm Teleprompter Plain One Way Mirror Glass Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

Whitaker,J.R.(1963),Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price. Analytical Chemistry35(12), 1950-1953[Hand Tools 9PCS Hex Key Set CRV Allen Wrench]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

3phase DC to AC Variable Frequency Drive Water Solar PV InverterAuthor for correspondence