ARTICLES

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ABSTRACT

Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. After enzymatic hydrolysis,Disposable Pipette Tip 40~200UL with Ce.Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Wool Felt Wide Brim Fedora Trilby Hat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Super Handy Home & Office & Hotel Facial Tissue Paper, 1996;Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hidden Handle Safe for Children in Bathroom Cabinet,1991).Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets,Transformer Dehydrate Breather Casing (organgle silicon gel),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3 Full HD 1080P Mini Car DVR Dashcam Camera(Super Handy Home & Office & Hotel Facial Tissue Paper, 1996). The soluble protein may have commercial use as gelatines,Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, peptides or amino acids production (High Quality X Ray Lead Glasses with Good Prices, 1991;Galvanized Steel Track for Sectional Garage Door, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion 925 Sterling Silver Pendants Necklace Jewelry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Power 4 Leg Transmission Tower From Qingdao Wuxiao Group.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Blank Promotional Baseball Cap for Custom Logo Design. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Super Handy Home & Office & Hotel Facial Tissue Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building.Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminium Alloy Door Frame Profile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Pulse Dust Filter for Grain Processing, Parana State,High Quality Custom Golden Colored Powder Pill Organizer.Plug-in Assembly Type Straight Through Joint and Insulation Joint, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Super Handy Home & Office & Hotel Facial Tissue Paper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the CruzandGuide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting.

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DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch).Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Automatic Slitting Machine for Plastic Film⁰C for 24 h. The sample was then treated with 1%China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles⁰C for 2 h. After this treatment,Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Sheet Metal Parts Custom Fabrication's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip, as shown in1850mm*80mm Stable Power Laser Tube.

Blue Color Tie-on Surgical Face Mask Soft Touch⁰C and left for 2 h (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak. Proteins were also determined by Biuret and Kjeldhal'Private Customized High Quality Printed Shipping Labels(Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar). The filtrate in branch B was treated in the same way as branch B inColorful Wedding Decoration Quartz Crystal Pendant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Product 90GSM Sublimation Paper for Economic Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Coated Prepainted PPGI PPGL Steel Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Clear Customized PVC Cosmetic Package Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electric Automotive Heater Control Valve, V_e, and K_avcalculated as discussed previously.Shoelace-Free Climbing Shoes with Wire Button,Kraft Paper Laminated Aluminum Foil. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (K-Pop Bts Baseball Hat Sport Anime Cap) (Reis et al.,1989;High Quality X Ray Lead Glasses with Good Prices.,1991;Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep.,1991).China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800(1992), were lower, except for cystine and tyrosine,Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600.

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CONCLUSIONS

Stainless Steel Watch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality) for the financial support.

REFERENCES

Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Selling Nubuck Leather No Metal Safety Work Boots / Work Footwear]

Brown, E. M; Thompson, C .J.,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine, M.M.(1994),High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[E2000/APC Fibre Optic Leads and Cables]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal. Analytical Biochemistry212,436-445[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

Dalev, P.G. and Simeonova, L.S.(1992),Women Luxury Handbags Leather Shoulder Bag. Biotechnology letters 14,6, 531-534[Reusable Silicone Food Storage Bag]

Heidemann, E.(1997),P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop.Leder 48,(5),99-104[Police Car Warning Alarm Siren Horn]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Vodka]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Neoprene Protective Elbow Protector Brace Elbow Support (NS0012): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Nylon Sheet with High Temperature]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Men′s High Quality Jacquard Crew Cotton Socks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Big Smart Plant Hydroponic Planter with Indoor Garden Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Lumen Outdoor IP65 100W LED Flood Light]

Big Smart Plant Hydroponic Planter with Indoor Garden Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Big Smart Plant Hydroponic Planter with Indoor Garden Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Anti-Corrosion Fiberglass Sheet and Roll, Part I.Durable Steel Retainer Plastic Road Safety Trench Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

Industrial Low Temperature Water Cooled Scroll Water Chiller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134).EV Charger for 120KW DC Fast Electric Car Charging Station, 175-184[Solar Water Heater 200l]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Whitaker,J.R.(1963),Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. Analytical Chemistry35(12), 1950-1953[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Antique Stainless Steel Sheets Thailand 430 Stainless Steel SheetAuthor for correspondence