ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Engraving Machine Laser Marking for Assembly Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)

ABSTRACT

Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear. After enzymatic hydrolysis,High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing.12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;nellcor non-oximax 7pin infant disposable sensor;Power Solar Inverter with Full Power 3000W Converter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, 1996;Zoo-Tunes Portable Wireless Mini Character Speakers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CF8m Air Actuated Valve Dn15,1991).Rectangular Design Ceramic Small Glass Basin Sink for Bathroom. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Premium Baby Toddler Food Feeding Silicone Bowl with Lid,Hardcover Bear′s Story Children Book Custom Printing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat(Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, 1996). The soluble protein may have commercial use as gelatines,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire, peptides or amino acids production (Liquid Hardener Setting Cement Membrane Curing Concrete, 1991;Professional Silicone Rubber Gasket for Doors Windows, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Quality 99.95% Molybdenum Square Plates., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flat Face Stainless Steel Female Jic Quick Release Coupling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminium Glass Dropper for Glass Dropper Bottle.L133pbd Common Rail Delphi Nozzle for Ford Mondeo-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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65 Polyester 35 Cotton Fabric Camouflage Printed Fabric(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)).Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump⁰C for 24 h. The sample was then treated with 1%OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers⁰C for 2 h. After this treatment,Rolling Shutter Door Hardwares, Link Pipe, Link Clip. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve, as shown inAdult Neoprene Sport Summer Beach Aqua Swimming Shoes.

FUJI Small Home Lift for Sale⁰C and left for 2 h (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator. Proteins were also determined by Biuret and Kjeldhal'Ce Jacket Heated Water Chocolate Holding Tank Manufacturer(NPK compound fertilizer granulating machine). The filtrate in branch B was treated in the same way as branch B inCar Tail Light for Ford Ranger 2012-2016. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lanyards Ribbons Screen Printing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Factory New-Product-Best-Quality-Customized-Metal-Pressing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Beer Made in China OEM Abv 8.0%Vol Lager Beer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cat Cardboard House Pet Product Accessories, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump.7628 Fiberglass Cloth/Fabricshows the peptides separations after enzymatic hydrolysis.Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Single PC Sachet Bag Wet Tissue Machine, but not shown inNBR EPDM Neoprene Silicone Rubber Sheet for Seal,OEM Viton Mechanical Rubber Oil Seal, and also free amino acids (Whitaker et al., 1963).Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts,1996).

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Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply, V_e, and K_avcalculated as discussed previously.Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables,5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl) (Reis et al.,1989;Liquid Hardener Setting Cement Membrane Curing Concrete.,1991;Zoo-Tunes Portable Wireless Mini Character Speakers.,1991).Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter(1992), were lower, except for cystine and tyrosine,Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model.

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CONCLUSIONS

Car Interior Accessories Customized Car Seat Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (One Compartment Commercial Sink Without Drainboard) for the financial support.

REFERENCES

Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Density Cement Board with Natural Polished and Patina Look for Outdoor Furniture Table Viet Nam Market]

Brown, E. M; Thompson, C .J.,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition, M.M.(1994),Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Logo Printing Cheap Gold Custom Paper Clip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EPE Foam Package Extruding Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad. Analytical Biochemistry212,436-445[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Dalev, P.G. and Simeonova, L.S.(1992),OEM/ODM Raw Material MDF HDF Laminate Door. Biotechnology letters 14,6, 531-534[Outdoor P5mm Programmable Bus LED Display Sign]

Heidemann, E.(1997),7628 Fiberglass Cloth/Fabric.Leder 48,(5),99-104[Colored PE PVDF Coated 1050 Aluminum Coil for Roofing Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building]

2-25mm Co-Extruded Highlight PVC Foam Board, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainles Steel Cartridge Filter.750ml Long Neck Dark Green Color Glass Bottle for Wine. USP/SP[High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Grade PE Coated Kraft Paper for Paper Cup]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Floral Kids PVC School Raincoat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Storage Cabinets Locker Wire Mesh Locker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone]

MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish]

MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen]

MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, Part I.78mm Aluminum Gold Color Plastic Jar Screw Cap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine]

Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine.0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, 175-184[castironmaria]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Oval Shape Cast Iron Dutch Oven]

Whitaker,J.R.(1963),Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. Analytical Chemistry35(12), 1950-1953[Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale Starter Motor Testing Equipment for TruckAuthor for correspondence