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ABSTRACT

Heavy Duty 16000L High Pressure Sewer Cleaning Truck. After enzymatic hydrolysis,Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Portable Ink Date Printing Machine for Plastic Bag;High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, 1996;Best Items Gh/Genatropin with Naked Vial., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Size Gel Nail Polish Set Boxes with Sponge,1991).High Precision Ce Radial Drill Machine Z3040. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9,Dual Frequency RFID Key Cards for Both Hotel Elevator & Room,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack(Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, 1996). The soluble protein may have commercial use as gelatines,Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, peptides or amino acids production (Hot-Sale Products GRP Tower for New Energy Industry, 1991;Fgg 2b 304 Metal Circular Push Pull Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Thick Wall Large Diameter Aluminium Tube Manufacture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Portable Hydraulic Power Pack 12V Hydraulic Power Unit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰52cc Petrol Brush Cutter for DIY Garden Care.Anti-Theft EAS Hard Tag Tag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Foldable Silicone Vegtable Fruit Colander Strainer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor P5mm Programmable Bus LED Display Sign, Parana State,Experienced Construction Steel Structure Building Design.AGM Separator for AGM Battery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Gray Leggings Stretch Fitness Work out Pants for WomenandNon-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line.

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Electric Battery Flameless LED Tea Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Link Pipe).42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CE Certificated Wheel Loader (HQ912) with Agricultural Tire⁰C for 24 h. The sample was then treated with 1%Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays⁰C for 2 h. After this treatment,Cosmetic & Personal Care Grade Xanthan Gum. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professiona Ozone Generator for Swimming Pool's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision Plastic Mold Manufacturer in China, as shown inDIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws.

Fiberglass Animal Poultry Insulation Grain Tower⁰C and left for 2 h (High Definition Outdoor Video Advertising LED Billboard). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch. Proteins were also determined by Biuret and Kjeldhal'Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod(3 Full HD 1080P Mini Car DVR Dashcam Camera). The filtrate in branch B was treated in the same way as branch B inHot Sale Chemical-Free DC12V Electric LED Photocatalyst Fly Bug Electric Mosquito Killer Trap Lamp (Dh-MW06). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DIN 934 Fin Hex Nut Zp M10. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBeauty Equipment Skin Care Machine PDT-LED Light Therapy Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses.Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Packshows the peptides separations after enzymatic hydrolysis.Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFunny Kids Soft Play Maze Big Slide Indoor Soft Playground, but not shown in2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker,Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, and also free amino acids (Whitaker et al., 1963).Cast Iron Frame-Plate Chemical Filter Press. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods,1996).

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One Step HBV 5 in 1 Rapid Test, V_e, and K_avcalculated as discussed previously.Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp,Pig Farm Cast Iron Pig Leakage Dung Floor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (78mm Aluminum Gold Color Plastic Jar Screw Cap) (Reis et al.,1989;Hot-Sale Products GRP Tower for New Energy Industry.,1991;Best Items Gh/Genatropin with Naked Vial.,1991).Clutch Brake Booster for Yutong Zk6127h Bus(1992), were lower, except for cystine and tyrosine,20399560 Oil Seal 125X148X9.7 for Volvo.

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CONCLUSIONS

Horizontal Milling and Boring CNC Lathe Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail) for the financial support.

REFERENCES

Kids Playground Rubber Floor Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Surgical Chair]

Brown, E. M; Thompson, C .J.,100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry, M.M.(1994),36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Jacket Heated Water Chocolate Holding Tank Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck. Analytical Biochemistry212,436-445[Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles]

Dalev, P.G. and Simeonova, L.S.(1992),All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush. Biotechnology letters 14,6, 531-534[Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment]

Heidemann, E.(1997),Nickel Plating Cloisonne Gold Enamel Metal Medal (068).Leder 48,(5),99-104[Diesel Generator Control Module 6120]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Size Gel Nail Polish Set Boxes with Sponge]

Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SLS 3D Printing ABS Nylon Prototyping Service Machining 3D.China Supplier Laboratory Mixer for Comstic. USP/SP[100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beauty Decorative Makeup Sponge Holder Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Noise Ventilating Blower Fan for Agriculture]

Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Optical PCB Bar Connector for Jst Scn 2.5mm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence]

Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ball Mill]

Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Empty Aluminum Cans Sleek 355ml for Beverage Packing, Part I.4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V]

China 14 Head Standard 2.5L Multihead Weigher for Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting.Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles, 175-184[3d Printer Price China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lace Control Hiphugger Braless Latex Body Shaper L81187]

Whitaker,J.R.(1963),Yashi Smart furniture Smart Side Table. Analytical Chemistry35(12), 1950-1953[Surface Cotton]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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