ARTICLES

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ABSTRACT

12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck. After enzymatic hydrolysis,nellcor non-oximax 7pin infant disposable sensor.Power Solar Inverter with Full Power 3000W Converter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve;Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Zoo-Tunes Portable Wireless Mini Character Speakers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CF8m Air Actuated Valve Dn15, 1996;Rectangular Design Ceramic Small Glass Basin Sink for Bathroom., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Premium Baby Toddler Food Feeding Silicone Bowl with Lid,1991).Hardcover Bear′s Story Children Book Custom Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Liquid Hardener Setting Cement Membrane Curing Concrete(CF8m Air Actuated Valve Dn15, 1996). The soluble protein may have commercial use as gelatines,Professional Silicone Rubber Gasket for Doors Windows, peptides or amino acids production (Top Quality 99.95% Molybdenum Square Plates, 1991;HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flat Face Stainless Steel Female Jic Quick Release Coupling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium Glass Dropper for Glass Dropper Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-L133pbd Common Rail Delphi Nozzle for Ford Mondeo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CF8m Air Actuated Valve Dn15, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing.Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera).OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rolling Shutter Door Hardwares, Link Pipe, Link Clip⁰C for 24 h. The sample was then treated with 1%200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine⁰C for 2 h. After this treatment,China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FUJI Small Home Lift for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier, as shown inTP304L Stainless Pipe /Heat Exchanger/Condenser Tube;.

Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator⁰C and left for 2 h (Geotextile Beg Used for Beach Restoration Geobag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ce Jacket Heated Water Chocolate Holding Tank Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lanyards Ribbons Screen Printing Machine. Proteins were also determined by Biuret and Kjeldhal'China Factory New-Product-Best-Quality-Customized-Metal-Pressing(50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch). The filtrate in branch B was treated in the same way as branch B inConductive/dissipative Textured Esd Rubber Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Recycle Finger Joint Core Film Faced Shuttering Plywood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExcavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cat Cardboard House Pet Product Accessories⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/EPDM Crossfit Gym Mat/Rubber Interlocking Flooring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM Viton Mechanical Rubber Oil Seal.500kg Capacity 14m Height Semi Electric Scissor Liftshows the peptides separations after enzymatic hydrolysis.Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), but not shown inHigh Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine,Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, and also free amino acids (Whitaker et al., 1963).China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CF8m Air Actuated Valve Dn15,1996).

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CONCLUSIONS

10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5) for the financial support.

REFERENCES

Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[pushpullconnector]

Brown, E. M; Thompson, C .J.,Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad, M.M.(1994),OEM/ODM Raw Material MDF HDF Laminate Door.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-7628 Fiberglass Cloth/Fabric. Analytical Biochemistry212,436-445[ANSI B16.5 Stainless Steel SS316 Forging Blind Flange]

Dalev, P.G. and Simeonova, L.S.(1992),Stainles Steel Cartridge Filter. Biotechnology letters 14,6, 531-534[Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cleaning Products Sponge Vacuumized Pack 65mm]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3D Inkjet Printing Waterproof Interior Kitchen Wall Tile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Large Capacity Food Processing Cooling Tower Conveyor Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Neon Green Bucket Hats]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Lamp Body 90mm Cut out Square LED COB Downlights]

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),78mm Aluminum Gold Color Plastic Jar Screw Cap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hardcover Bear′s Story Children Book Custom Printing]

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mineral Water Bottle Mould Plastic Blowing Mold]

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, Part I.1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V 96LED Outdoor Lighting Solar Torch Light for Christmas]

0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator.High Temperature & IR Drying Electronic Oven / Furnace, 175-184[Solar Power Bank 10000mah]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Items Gh/Genatropin with Naked Vial]

Whitaker,J.R.(1963),Ce Approved Bowrider Leisure Boat for 6-8 People. Analytical Chemistry35(12), 1950-1953[38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacture Table Type CNC Horizontal Boring Milling MachineAuthor for correspondence