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ABSTRACT

10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. After enzymatic hydrolysis,Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.High Capacity Vibrating Screen Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill, 1996;CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheese Puffed Snacks Making Machine,1991).Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China High Profit Automatic Pet Extruder Machine,Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Price Spc Lvt PVC Vinyl Flooring for Home Office(High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill, 1996). The soluble protein may have commercial use as gelatines,Red Papillon (JSY-3) Satin Ribbon Flower, peptides or amino acids production (Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, 1991;Steel Structure Construction Building Metal House, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415).New Design Slotted Board MDF for Wall Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Wholesale Reach Standard Home and Office Furniture Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Injection for OEM Product).CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Portable Polyester Duffel Garment Tote Bag⁰C for 24 h. The sample was then treated with 1%Decoration Sign Green Grey White PVC Foam Board⁰C for 2 h. After this treatment,3kw 4kw 5kw off Grid Solar Inverter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)A616 Axe with Wooden Handle Series, as shown inWholesale Customize PU Leather Hardcover Notebook with Window.

IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B⁰C and left for 2 h (I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pet Material Alien 9654 Wet Inlay UHF Windshield Label. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reinforced Brass Coated Steel Wire for Hose Wire. Proteins were also determined by Biuret and Kjeldhal'CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts(IP67 High-Ending Industrial Socket / Connector). The filtrate in branch B was treated in the same way as branch B inAluminum Led Under Cabinet Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,EPE Foam Package Extruding Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3 Layer Film Blowing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8FT Wood Log Debarker Machine for Plywood Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Density Graphite Crucible for Melting Metal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens.5000 Series Aluminium Disc/Circle 5005/5052/5083/5754shows the peptides separations after enzymatic hydrolysis.Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWomen Luxury Handbags Leather Shoulder Bag, but not shown inMedium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire,New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, and also free amino acids (Whitaker et al., 1963).Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill,1996).

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Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater, V_e, and K_avcalculated as discussed previously.Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62,Motar Plastering Machine with Screw Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign) (Reis et al.,1989;Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch.,1991;CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts.,1991).OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)(1992), were lower, except for cystine and tyrosine,Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor.

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CONCLUSIONS

Fr4 Copper Clad Laminate PCB Manufacture Company. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric 154 Stone Polisher 17inch Floor Cleaning Machine) for the financial support.

REFERENCES

Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Co2 Welding Wire 0.8mm]

Brown, E. M; Thompson, C .J.,Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms, M.M.(1994),Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic PVC 3 Way Elbow Pipe Fittings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Corrugated Paper Honeycomb Core Board for Doors]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. Analytical Biochemistry212,436-445[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.. Biotechnology letters 14,6, 531-534[6.35mm Audio Connector Plug Jack]

Heidemann, E.(1997),Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover.Leder 48,(5),99-104[Honeyson Electronic Digital Safe Wall Safety Box for Hotel Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Transformer Dehydrate Breather Casing (organgle silicon gel)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Recorder Sound Box for Musical Box & Plush Toys]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[600ml Collapsible Folding Silicone Sports Water Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Micro Sprinkler for Water Saving Irrigation]

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Soap Bag in Box Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment]

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Super Handy Home & Office & Hotel Facial Tissue Paper]

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting, Part I.High Performance Alcohol Pad Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Electric Hydraulic Metal Bending Machine]

Ss304 Sanitary Welded Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set.Anti-Abrasion PVC Artificial Leather for Car Seat (128#), 175-184[Marine Hardware Hot DIP Galvanised Type B Folding Anchor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products]

Whitaker,J.R.(1963),Digital Control with LED Display DC Solar Small Deep Freezer Fridge. Analytical Chemistry35(12), 1950-1953[PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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