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ABSTRACT

Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. After enzymatic hydrolysis,Metal Unfolding Grocery Push Carts.Auto/Manual Spray Painting Booth/Cabinet with Best Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m;2019 Dotted Navy Rucksack Children School Bag Cotton Backpack; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Multi Span Polycarbonate Industrial Green House, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Plastic Injected (injection) Parts Molding Process, 1996;4mm 6mm2 Soler Extension Leads with Mc4., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (E0303 300n Activator Chiropractic Impulse Adjusting Instrument,1991).Men′s Black and Grey Stripe Bamboo Dress Sock. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part,China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Mobile Phone Shop Display Furniture Interior Design(Custom Plastic Injected (injection) Parts Molding Process, 1996). The soluble protein may have commercial use as gelatines,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market, peptides or amino acids production (Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1991;Rubber Seal for Crank Box of Hks Warp Knitting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Plastic Injected (injection) Parts Molding Process, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine.Extruded Polystyrene Foam Board Custom Edges S/L-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Commercial Coca Cola Refrigerator with Glass Door, Parana State,Essence Oil Amber Glass Bottle with Plastic Dropper.Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Plastic Injected (injection) Parts Molding Process, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYgk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinetand35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller.

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Double Jacketed Cooking Kettle Electric Jacket Boiler(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashionable Simple Printing Mini Key & Coin Purse).Fashion Gray Leggings Stretch Fitness Work out Pants for Women(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules⁰C for 24 h. The sample was then treated with 1%High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve⁰C for 2 h. After this treatment,Medical Grade Ventilator for Sleep Apnea Breathing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)UV Resistant Single Component Water Based Polyurethane Waterproof Coating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel Rubber Seal for Dust Proof, as shown inTop Stainless Steel Baluster Designs Stair Baluster Railing Handrail.

Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf⁰C and left for 2 h (Stainless Steel Automatic Water Flow Control Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kindergarten Playground Preschool Kids Plastic Slide and Swing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass. Proteins were also determined by Biuret and Kjeldhal'COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery(Concrete Electric Pole Pile Making Spinning Machine). The filtrate in branch B was treated in the same way as branch B inBroiler Farm Design. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCapacitive Push Button Membrane Keyboards(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Used Tyre Recycling Plant for Reclaimed Rubber ProductionandManufacturer Custom DSLR SLR Camera Sling Backpack with Zipper. Enzymatic (12V 96LED Outdoor Lighting Solar Torch Light for Christmas)Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line(sulfuric acid)SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa(Custom Plastic Injected (injection) Parts Molding Process, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Efficiency Swimming Pool Air Source Water Heat Pump, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Efficiency Swimming Pool Air Source Water Heat Pump, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service.Children Shoulder Backpack Bag Leisure School Bagshows the peptides separations after enzymatic hydrolysis.Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 16.9r38 Dong Ah Brand, but not shown inTungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking,Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, and also free amino acids (Whitaker et al., 1963).QJ-M Moving small manual brick making machine with low cost. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Plastic Injected (injection) Parts Molding Process,1996).

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PVC Hdo Construction Formworks, V_e, and K_avcalculated as discussed previously.Screw Dosing Feeder Used for Chicken Feed Plant,5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry) (Reis et al.,1989;Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer.,1991;4mm 6mm2 Soler Extension Leads with Mc4.,1991).Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile(1992), were lower, except for cystine and tyrosine,Automatic Waste Paper Stripper.

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CONCLUSIONS

Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery) for the financial support.

REFERENCES

Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Boys Drop Light Shade High Quality Supplier]

Brown, E. M; Thompson, C .J.,Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1, M.M.(1994),U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud.The Journal of the American Leather Chemists Association (JALCA)89(7)[3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi. Analytical Biochemistry212,436-445[Supermarket Combined Island Chest Freezer Commercial Refrigerator]

Dalev, P.G. and Simeonova, L.S.(1992),99% Purity Caustic Soda Flakes From Direct Manufacture on Sale. Biotechnology letters 14,6, 531-534[OEM Viton Mechanical Rubber Oil Seal]

Heidemann, E.(1997),Bwb-306 Custom Printing Outdoor Sports Wristband.Leder 48,(5),99-104[Eco Cellulose Scrub Sponges Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch]

Automotive ECU Wire Harness 24 Pin Grey Connector, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Irregular Shape Hologram Label Printing.High Speed Rope Weaving Machine. USP/SP[Cummins L10 Engine Piston Cooling Nozzle 3070218]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Natural Clean Non- Ornamentate Decoration Material Wallpaper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Special Design High Quality Custom Running Sport Marathon Finisher Medal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)]

Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lanyards Ribbons Screen Printing Machine]

Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Seamless Boxer Brief for Men]

Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, Part I.Large Prototype CNC Transmission Assembly Tractor Machinery Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle]

China Durable 25kg 50kg Red PP Woven Bag Sack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock.Mini Recorder Sound Box for Musical Box & Plush Toys, 175-184[www.packagingbagfactory.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Apex Digital UV Printers for Aluminum Board Printing UV6090]

Whitaker,J.R.(1963),Fake Fish Luminescent Tropical Fish Fish Tank Decoration. Analytical Chemistry35(12), 1950-1953[Auto Valve 4-20mA Flow Control Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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