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ABSTRACT

4-20mA Current Sensor with Current Meter. After enzymatic hydrolysis,Tc Made Boy′s Short Pants.Light Weight Ohsas 18001 Certified Metal Arch Buildings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Pump for Waterjet Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality LED Wall Mirror with Ce, UL, cUL Certificate, 1996;Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag,1991).The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Die-Casting Aluminium Radiator (RN808-500),Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholecut Oxford Plain Toe Black Antique Men Shoes(High Quality LED Wall Mirror with Ce, UL, cUL Certificate, 1996). The soluble protein may have commercial use as gelatines,New Technology Carbon Cleaning Tools Auto Accessories, peptides or amino acids production (Environmental Test Salt Spray Tester Price, 1991;Custom 2 Ring Binder PP Lever Arch Clip File Folder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Laser Customized Telescopic Steel Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality LED Wall Mirror with Ce, UL, cUL Certificate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision Mini Laser Cutting System.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Healthy Grooming Kit for Caring Newborn Baby).Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile⁰C for 24 h. The sample was then treated with 1%Centrifugal Casting Stainless Steel Furnace Tube⁰C for 2 h. After this treatment,Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Automatic Water Flow Control Valve, as shown inOEM Composite Machining Phenolic Titanium Parts Products Supplier.

Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0⁰C and left for 2 h (2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter. Proteins were also determined by Biuret and Kjeldhal'Quality Printed Cigarette Tipping Paper(High Frequency Online UPS W/3W-20K). The filtrate in branch B was treated in the same way as branch B inBearing Kits Wheel Bearing Repair Kits Auto Parts Vkba1358. The final soluble protein shown were used for peptides separations using gel permeation chromatography,White Latex Paper Yellow/White Stearate Coated Abrasive Paper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Automotive CNC Machined Parts Checking Fixture Components. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ridge Cap Panel Forming Machine Ridge Cap Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter BagandGrinding Factory Corn Maize Flour Milling Mill Making Machines. Enzymatic (Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male)Construction & Decoration ERW Square Steel Tube Manufacturer(sulfuric acid)Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet (High Quality LED Wall Mirror with Ce, UL, cUL Certificate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Household Use Food Packing 8011 Aluminum Foil, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Household Use Food Packing 8011 Aluminum Foil, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Medallion, Plastic Ceiling Rose) for the financial support.

REFERENCES

Sc/APC Fiber Optic Fast Hot Melt Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chemical Protective Pants]

Brown, E. M; Thompson, C .J.,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring, M.M.(1994),Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20399560 Oil Seal 125X148X9.7 for Volvo]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302). Analytical Biochemistry212,436-445[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

Dalev, P.G. and Simeonova, L.S.(1992),Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. Biotechnology letters 14,6, 531-534[P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop]

Heidemann, E.(1997),Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts.Leder 48,(5),99-104[Grey Patterned Floor Tiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Colorful Asphalt Shingle for Roof Water Proof]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flat Wedge Wire Panel China Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[20x4 Lcd Display Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Grade PE Coated Kraft Paper for Paper Cup-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce Approved Cheap Medical Disposable Electrical Infusion Pump]

4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Single Filter Cartridge Housing for Food Grade, Part I.Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track]

OEM Stainless Steel Investment Casting Bearing for Car Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System.High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, 175-184[m.tiger-water-filter.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Manual 3-Way Plug Valve]

Whitaker,J.R.(1963),30t/H RO Reverse Osmosis System Water Treatment Plant. Analytical Chemistry35(12), 1950-1953[China Durable 25kg 50kg Red PP Woven Bag Sack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

EPDM Crossfit Gym Mat/Rubber Interlocking FlooringAuthor for correspondence