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ABSTRACT

0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer. After enzymatic hydrolysis,Bgf-800 Pet Dry Laminating Machine.Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging;Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Polyester Cocoon Bobbins Thread for Household Textiles, 1996;Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (HAIRUI 850A+ 600W SMD Rework Soldering Machine,1991).High Quality Oval Shape Cast Iron Dutch Oven. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara,Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109(Polyester Cocoon Bobbins Thread for Household Textiles, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, peptides or amino acids production (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, 1991;High Performance Fresh Air Handling Unit for Marine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Community IC Access Card Induction ID Time Card Smart Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Packing Machine Biscuit Packing Machine with Auto Feeder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Polyester Cocoon Bobbins Thread for Household Textiles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Military Uniform ISO and SGS Standard for Army Camouflage.300W-2kw Solar Panel and Wind Turbine Hybrid System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Good Quality High Precision Machined Milling CNC Brass Parts, Parana State,Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine.Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Polyester Cocoon Bobbins Thread for Household Textiles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePig Farm Cast Iron Pig Leakage Dung FloorandBoiler Room Ventilation Exhaust Fan.

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Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1300 Paper Slitting and Rewinding Machine).5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Single PC Sachet Bag Wet Tissue Machine⁰C for 24 h. The sample was then treated with 1%Aluminum Road Stud⁰C for 2 h. After this treatment,Lab Water Instruments/Lab Water Purification System Micro. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, as shown inHigh Quality X Ray Lead Glasses with Good Prices.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBitumen Waterproof Membrane Minerla Surface Waterproof Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pfc Inductors with Adjustable Dimension and Wire Diameter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Permanent Magnetic Lifter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Diameter Aluminum Pipe 3004, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar LampandHpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. Enzymatic (Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater)Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine(sulfuric acid)Wholesale Logo Printing Cheap Gold Custom Paper Clip(Polyester Cocoon Bobbins Thread for Household Textiles, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;, flow rate of 0.1 mL/min., and l = 620 nm.

Non - Smell Fiber Reinforced PVC Garden Hose.8FT Wood Log Debarker Machine for Plywood Machineryshows the peptides separations after enzymatic hydrolysis.PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in30t/H RO Reverse Osmosis System Water Treatment Plant, but not shown inTop Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector,Boiler Room Ventilation Exhaust Fan, and also free amino acids (Whitaker et al., 1963).Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Polyester Cocoon Bobbins Thread for Household Textiles,1996).

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New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain, V_e, and K_avcalculated as discussed previously.New 14 Inch Diamond Saw Blade for Granite Cutting,TV Antenna (CH50006). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Speed Rope Weaving Machine) (Reis et al.,1989;1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.,1991;Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood.,1991).4PCS High Quality Adjustable Wrench Set (FST-41)(1992), were lower, except for cystine and tyrosine,Three F Female Connector Adapter.

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CONCLUSIONS

Tongkai Classic Business Hardcover Leather Notebook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (RV UPS Power 48V 80ah LiFePO4 Battery Pack) for the financial support.

REFERENCES

Customization! Bdy380CD Horizontal Directional Drilling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.pengtongfabric.com]

Brown, E. M; Thompson, C .J.,500kg Capacity 14m Height Semi Electric Scissor Lift, M.M.(1994),Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Printed Cigarette Tipping Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen. Analytical Biochemistry212,436-445[CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose. Biotechnology letters 14,6, 531-534[Industrial Bedsheet Folding Machine (ZD-3000)]

Heidemann, E.(1997),Small Batch Processing Football Net.Leder 48,(5),99-104[Stainless Steel Diced Meat Machine/Meat Slicer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[National Day Holiday Football Bravo Hand Weaving Flag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bell Transformer Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hand Tools 9PCS Hex Key Set CRV Allen Wrench]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SBR Round DOT Horse Stall Mat, Rubber Stable Mat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3phase DC to AC Variable Frequency Drive Water Solar PV Inverter]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Ce Radial Drill Machine Z3040]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Frame Mount Highway Pegs Enclosure Parts, Part I.Double Wine Carrier Bag Gift Box Tote. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor]

IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls.RF Coaxail BNC Male to BNC Male Plug Connector Adapter, 175-184[SC Male to LC Female Simplex Fiber Optic Adapters]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304/304L 2b Stainless Steel Sheet and Steel Plate]

Whitaker,J.R.(1963),Gt4b63 Can Seamer Machine. Analytical Chemistry35(12), 1950-1953[Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std SizeAuthor for correspondence