ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. After enzymatic hydrolysis,Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C).Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outdoor LED Decorative Street Linear Wall Lighting;Good Quanlity of Ue319an20z Hydraulic Oil Filter Element; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Universal Manual Turret Milling Machine(Mill X6336), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1996;Hexagonal Netting/ Gabion Box/ Gabion Basket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Proper Price Good Quality 10ml 20ml Airless Pump Bottle,1991).BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fire Retardant Reflective Aluminium Foil Woven for Insulation Material,Fireproof WPC Indoor Decorative Wall Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch(Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1996). The soluble protein may have commercial use as gelatines,3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, peptides or amino acids production (High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine, 1991;CNC Machinery Aluminum and ABS Mock up Prototypes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bsp 60° Male Cone Seat Carbon Steel Fittings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Soccer Field Artificial Grass Cutting Machine for Installation.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Nylon Sheet with High Temperature. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC TPU Tr 2 Color Sole Injection Moulding Machine.Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, Parana State,Flow Packing Machine for Bread, Soap, Biscuit, Chocolate.Disposable Pipette Tip 40~200UL with Ce, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1/2 Inch 4sh 4sp Steel Wire spiral High Pressure HoseandBoat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch.

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Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden).1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%86 Type WiFi Smart Touch Switch Controll by Tuya APP⁰C for 24 h. The sample was then treated with 1%100% Wool Felt Wide Brim Fedora Trilby Hat⁰C for 2 h. After this treatment,Super Handy Home & Office & Hotel Facial Tissue Paper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hidden Handle Safe for Children in Bathroom Cabinet, as shown inWholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar.

Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale⁰C and left for 2 h (Diamond Cutting Blades for Asphalt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Transformer Dehydrate Breather Casing (organgle silicon gel). Proteins were also determined by Biuret and Kjeldhal'3 Full HD 1080P Mini Car DVR Dashcam Camera(Wholecut Oxford Plain Toe Black Antique Men Shoes). The filtrate in branch B was treated in the same way as branch B inAutomatic Fabric Laser Cutting Machine Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality X Ray Lead Glasses with Good Prices. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Steel Track for Sectional Garage Door(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Power 4 Leg Transmission Tower From Qingdao Wuxiao Group⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Blank Promotional Baseball Cap for Custom Logo Design, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality 3000L Composting Machine with Lower PriceandFive Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine. Enzymatic (Aluminium Alloy Door Frame Profile)M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6(sulfuric acid)High Quality 98% Feed Grade Betaine Anhydrous(Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wood Handle Carbon Steel Sickle S202) for the financial support.

REFERENCES

Private Customized High Quality Printed Shipping Labels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Public Transport Type Autowalk]

Brown, E. M; Thompson, C .J.,DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, M.M.(1994),2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen.The Journal of the American Leather Chemists Association (JALCA)89(7)[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reusable Silicone Food Storage Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Product 90GSM Sublimation Paper for Economic Printing. Analytical Biochemistry212,436-445[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting]

Dalev, P.G. and Simeonova, L.S.(1992),1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System. Biotechnology letters 14,6, 531-534[Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment]

Heidemann, E.(1997),Color Coated Prepainted PPGI PPGL Steel Sheet.Leder 48,(5),99-104[Insulated Cola Water Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Vodka]

Straight Seam Galvanized Pipe High Frequency Tube Welding Mill, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate.Clear Customized PVC Cosmetic Package Bag. USP/SP[Plastic Nylon Sheet with High Temperature]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Lumen Outdoor IP65 100W LED Flood Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Wing Box Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Factory Professional Design Wing Shape Engraved Car Label Emblem, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

Factory Professional Design Wing Shape Engraved Car Label Emblem,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Factory Professional Design Wing Shape Engraved Car Label Emblem, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Dual Voltage Steam Curling Tongs (A129), Part I.High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

ASME Butt Weld Seamless Carbon Steel Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.Two Wheel Foldable Electric Kick Scooter, 175-184[Qtm40-3A Egg Layer Manual Concrete Block Brick Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet]

Whitaker,J.R.(1963),Diamond Cutting Blades for Asphalt, Granite, Marble. Analytical Chemistry35(12), 1950-1953[99% Al2O3 High Alumina Ceramic Ball]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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