ARTICLES

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ABSTRACT

Custom Size Gel Nail Polish Set Boxes with Sponge. After enzymatic hydrolysis,High Precision Ce Radial Drill Machine Z3040.Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dual Frequency RFID Key Cards for Both Hotel Elevator & Room;NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot-Sale Products GRP Tower for New Energy Industry, 1996;Fgg 2b 304 Metal Circular Push Pull Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Thick Wall Large Diameter Aluminium Tube Manufacture,1991).Portable Hydraulic Power Pack 12V Hydraulic Power Unit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW,52cc Petrol Brush Cutter for DIY Garden Care,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti-Theft EAS Hard Tag Tag(Hot-Sale Products GRP Tower for New Energy Industry, 1996). The soluble protein may have commercial use as gelatines,Foldable Silicone Vegtable Fruit Colander Strainer, peptides or amino acids production (Hans Custom Manufactured Smoothness Zipper Waterproof, 1991;Quartz Liquid Powder Feeding Torch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor P5mm Programmable Bus LED Display Sign. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot-Sale Products GRP Tower for New Energy Industry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰AGM Separator for AGM Battery.Electric Battery Flameless LED Tea Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;).New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod⁰C for 24 h. The sample was then treated with 1%Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator⁰C for 2 h. After this treatment,DIN 934 Fin Hex Nut Zp M10. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz, as shown inGeotextile Beg Used for Beach Restoration Geobag.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Span Steel Structrure for Warehouse From China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Steel Prefab Layer Cage Chicken House Poultry Farm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Supplier Laboratory Mixer for Comstic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cosmetic & Personal Care Grade Xanthan Gum) for the financial support.

REFERENCES

Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Decoration Tabletop Flower Vase for Celebration Events]

Brown, E. M; Thompson, C .J.,Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, M.M.(1994),Optical PCB Bar Connector for Jst Scn 2.5mm.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ANSI B16.5 Stainless Steel SS316 Forging Blind Flange]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Empty Aluminum Cans Sleek 355ml for Beverage Packing. Analytical Biochemistry212,436-445[Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Capacity Food Processing Cooling Tower Conveyor Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yashi Smart furniture Smart Side Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Lamp Body 90mm Cut out Square LED COB Downlights]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Concrete Interlocking Block Machine High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hardcover Bear′s Story Children Book Custom Printing]

PP Base Packaging Film Pet/PE Laminating Food Packaging Film, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Professional Indoor&Outdoor Trampoline Park-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mineral Water Bottle Mould Plastic Blowing Mold]

PP Base Packaging Film Pet/PE Laminating Food Packaging Film,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V 96LED Outdoor Lighting Solar Torch Light for Christmas]

PP Base Packaging Film Pet/PE Laminating Food Packaging Film, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation, Part I.Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Items Gh/Genatropin with Naked Vial]

Air Cooled Heat Pump Chiller Can Be Customized, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),50d Cationic Spandex Stretch Fabric for Garment.Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), 175-184[Permanent Neodymium Bar Magnets Generators]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle]

Whitaker,J.R.(1963),Cavitation Vacuum RF Lipo Laser Slimming Machine. Analytical Chemistry35(12), 1950-1953[China Manufacture Table Type CNC Horizontal Boring Milling Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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