ARTICLES

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ABSTRACT

New Small Plug-in Insect Killer Mosquito Killing Lamp. After enzymatic hydrolysis,Wholesale Customize PU Leather Hardcover Notebook with Window.Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Composite Machining Phenolic Titanium Parts Products Supplier, 1996;DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing,1991).Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film,Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Xtsky Automotive Wheel Hub Bearing (DAC37720037)(OEM Composite Machining Phenolic Titanium Parts Products Supplier, 1996). The soluble protein may have commercial use as gelatines,Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle, peptides or amino acids production (Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, 1991;FTTH Optical Network Terminal for Gpon ONU and CATV, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Customized Polyester Cotton National President Election Supporters Printed Scarf., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.D8tc High Performance 3-Electrode Motorcycle Spark Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Composite Machining Phenolic Titanium Parts Products Supplier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging.Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:19m Mobile LED Display Billboard for Advertising Screens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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150 Ton Hydraulic Press Machine for Cooking Pot Making(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)).Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Flat New Design Ladies Winter Warm Shoes⁰C for 24 h. The sample was then treated with 1%Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves⁰C for 2 h. After this treatment,OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal, as shown inFashionable Clothing Garment Paper Swing Tags (ST-01).

IP67 High-Ending Industrial Socket / Connector⁰C and left for 2 h (Telescopic Slideway Steel Protection Accordion Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mc4 Solar PV Connector TUV Approval. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China. Proteins were also determined by Biuret and Kjeldhal'Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price(Galvanized Steel Barbed Wire Rope 8mm). The filtrate in branch B was treated in the same way as branch B inStainless Steel Dish Head For Conical Head. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Over Band Round Type Electro Magnetic Separator for Tramp Iron.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic China Beef Cattle Sheep Tripe Washer Cleaning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bgf-800 Pet Dry Laminating Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Oval Shape Cast Iron Dutch Oven.Plug-in Assembly Type Straight Through Joint and Insulation Jointshows the peptides separations after enzymatic hydrolysis.White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, but not shown in304 Seamless Stainless Steel Tube,Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, and also free amino acids (Whitaker et al., 1963).Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Composite Machining Phenolic Titanium Parts Products Supplier,1996).

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CONCLUSIONS

EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Home Machine 2019 New Commercial Treadmill Gym Equipment) for the financial support.

REFERENCES

Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Hair Tie]

Brown, E. M; Thompson, C .J.,Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track, M.M.(1994),RV UPS Power 48V 80ah LiFePO4 Battery Pack.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Good Quality High Precision Machined Milling CNC Brass Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lightweight Design Industrial Robot with Six Axis for Painting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Popular Cheap Custom Logo Black Silicone Wristband in High Quality. Analytical Biochemistry212,436-445[Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Good Quality High Precision Machined Milling CNC Brass Parts. Biotechnology letters 14,6, 531-534[Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier]

Heidemann, E.(1997),Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes.Leder 48,(5),99-104[Led Electronic Blackboard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bag Accessories 10mm Metal Dog Swivel Snap Hook]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Single PC Sachet Bag Wet Tissue Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100ml Plastic E Liquid Vape Cig Juice Dropper Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Airtight Storage Jar Shiny Herb Storage Jar 100ml/180ml]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Skateboard Shoes]

Aluminum Road Stud, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lab Water Instruments/Lab Water Purification System Micro-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brake Fluid Tank Container Hot Plate Plastic Welding Machine]

Aluminum Road Stud,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System]

Aluminum Road Stud, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia, Part I.Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Walkway]

Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lightweight Compact 70d Ripstop Nylon Parachute Hammock.0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, 175-184[Wall Mounted Socket]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

Whitaker,J.R.(1963),China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106). Analytical Chemistry35(12), 1950-1953[Aluminium Glass Dropper for Glass Dropper Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Professional Indoor&Outdoor Trampoline ParkAuthor for correspondence