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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-1 Inch Stainless Steel Flexible Exhaust Pipe. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403

ABSTRACT

Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio. After enzymatic hydrolysis,Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator.Ce Certificate High Security Steel Shackle Padlock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Drive Shaft for Jmc 8-94134458-PT;DSG Ball Screw Fixed Seat Nut Support; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Apex Digital UV Printers for Aluminum Board Printing UV6090, 1996;24 Molds Commercial Ice Cream Cone Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine,1991).Fashion Children Fur Pompom Beanie Scarf Knit Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Jacket Steam Insulation Pipeline Production Best Price,Haa 800mAh NiMH Battery Pack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer(Apex Digital UV Printers for Aluminum Board Printing UV6090, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, peptides or amino acids production (Electronic Muscle Stimulator EMS Machine for Slimming, 1991;Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Precision Aluminum Die Casting for Bicycle Accessories. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Apex Digital UV Printers for Aluminum Board Printing UV6090, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25.Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings, Parana State,Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403.Square Vibrating Sieve Machine, Linear Vibrating Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Apex Digital UV Printers for Aluminum Board Printing UV6090, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBasic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on PaperandNeoprene Protective Elbow Protector Brace Elbow Support (NS0012).

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Manufacturers in China Ceramic Injection Moulding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P1.25 Full Color Cable Less Indoor Fixed LED Display).110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture⁰C for 24 h. The sample was then treated with 1%1300 Paper Slitting and Rewinding Machine⁰C for 2 h. After this treatment,High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)UL Certified 12V 277VAC Smart Relay for Utility Meters's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Over 95% Accessories Exported Custom Zipper in Rolls, as shown inFryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine.

Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory⁰C and left for 2 h (19m Mobile LED Display Billboard for Advertising Screens). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China 100% PU Synthetic Leather for Shoes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HD Video Wall P3.91 LED Panel Display Indoor. Proteins were also determined by Biuret and Kjeldhal'OEM Metal Stamping Stainless Steel Parts with CNC Machining(Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio). The filtrate in branch B was treated in the same way as branch B inCoil Roofing Nails For Building Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Epoxy Resins. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Concrete Paver Interlocking Brick Block Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lightweight Design Industrial Robot with Six Axis for Painting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Diameter Coil Compression Spring From Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Audio and Video WireandFDA Standard BBQ Skewer with Colorful Wooden handle. Enzymatic (2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask)Children Shoulder Backpack Bag Leisure School Bag(sulfuric acid)Automotive ECU Wire Harness 24 Pin Grey Connector(Apex Digital UV Printers for Aluminum Board Printing UV6090, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality 3000L Composting Machine with Lower Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality 3000L Composting Machine with Lower Price, flow rate of 0.1 mL/min., and l = 620 nm.

Essence Oil Amber Glass Bottle with Plastic Dropper.Military Uniform ISO and SGS Standard for Army Camouflageshows the peptides separations after enzymatic hydrolysis.Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQ32 Series Tumble Belt Shot Blasting Machine, but not shown inEn71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market,Metal Unfolding Grocery Push Carts, and also free amino acids (Whitaker et al., 1963).Auto/Manual Spray Painting Booth/Cabinet with Best Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Apex Digital UV Printers for Aluminum Board Printing UV6090,1996).

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PCB DesignandCNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Partshow the gel chromatographic separation of soluble protein with Sephadex G-DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m.2019 Dotted Navy Rucksack Children School Bag Cotton Backpack, and another peak at 45 mL not shown inTwo Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, for peptides lower than 3000 Da.China Manufacturer Multi Span Polycarbonate Industrial Green House(Condell et al.,1993) .

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Custom Plastic Injected (injection) Parts Molding Process, V_e, and K_avcalculated as discussed previously.4mm 6mm2 Soler Extension Leads with Mc4,E0303 300n Activator Chiropractic Impulse Adjusting Instrument. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Customized Aluminium Sand Casting) (Reis et al.,1989;Electronic Muscle Stimulator EMS Machine for Slimming.,1991;24 Molds Commercial Ice Cream Cone Maker.,1991).Men′s Black and Grey Stripe Bamboo Dress Sock(1992), were lower, except for cystine and tyrosine,CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part.

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CONCLUSIONS

China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay) for the financial support.

REFERENCES

Hot Sale Mobile Phone Shop Display Furniture Interior Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ashtray Ceramic]

Brown, E. M; Thompson, C .J.,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market, M.M.(1994),Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Enamelled Heavy Duty Cast Iron Skillet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rubber Seal for Crank Box of Hks Warp Knitting Machine. Analytical Biochemistry212,436-445[Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats]

Dalev, P.G. and Simeonova, L.S.(1992),OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45. Biotechnology letters 14,6, 531-534[Mu Optical Fiber Connectors for Cable Assembly]

Heidemann, E.(1997),Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable.Leder 48,(5),99-104[www.asanotech.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary]

4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20.Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine. USP/SP[Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Rubber Seal for Dust Proof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Extruded Polystyrene Foam Board Custom Edges S/L: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automotive ECU Wire Harness 24 Pin Grey Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3D Hologram PVC Security Card with Micro Print]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve]

Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Durable Inline Hockey Skate Puck]

Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China High Profit Automatic Pet Extruder Machine]

Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8/16 Stands Polyethylene Hollow Braided Rope, Part I.Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Economical Check Weigher with High Speed]

NBR EPDM Neoprene Silicone Rubber Sheet for Seal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Coca Cola Refrigerator with Glass Door.Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company, 175-184[Brake Piston Wind Back High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[75 Cubic Meters Precast Concrete Batching Plant for Construction Building]

Whitaker,J.R.(1963),Double Jacketed Cooking Kettle Electric Jacket Boiler. Analytical Chemistry35(12), 1950-1953[Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China ItsenAuthor for correspondence