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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Single PC Sachet Bag Wet Tissue Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Road Stud

ABSTRACT

Lab Water Instruments/Lab Water Purification System Micro. After enzymatic hydrolysis,Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia.Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig;Lightweight Compact 70d Ripstop Nylon Parachute Hammock; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), 1996;Double Bowl Undermount for Stainless Steel Kitchen Sink., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport,1991).Bitumen Waterproof Membrane Minerla Surface Waterproof Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,M10 3D Solid Non-Woven Folded Mask Body Making Machine,Pfc Inductors with Adjustable Dimension and Wire Diameter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Permanent Magnetic Lifter(China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), 1996). The soluble protein may have commercial use as gelatines,Large Diameter Aluminum Pipe 3004, peptides or amino acids production (Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, 1991;Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Logo Printing Cheap Gold Custom Paper Clip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Non - Smell Fiber Reinforced PVC Garden Hose.PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Boiler Room Ventilation Exhaust Fan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain, Parana State,Aluminum Road Stud.New 14 Inch Diamond Saw Blade for Granite Cutting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Lumen Outdoor IP65 100W LED Flood LightandFiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector.

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TV Antenna (CH50006)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Price Spc Lvt PVC Vinyl Flooring for Home Office).4PCS High Quality Adjustable Wrench Set (FST-41)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Three F Female Connector Adapter⁰C for 24 h. The sample was then treated with 1%Tongkai Classic Business Hardcover Leather Notebook⁰C for 2 h. After this treatment,Customization! Bdy380CD Horizontal Directional Drilling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500kg Capacity 14m Height Semi Electric Scissor Lift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale, as shown in1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Frame Mount Highway Pegs Enclosure Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Wine Carrier Bag Gift Box Tote, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6shows the peptides separations after enzymatic hydrolysis.Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, but not shown inFull Automatic Combination Weighing Packing Machine Jy-420A,Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment, and also free amino acids (Whitaker et al., 1963).Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106),1996).

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CONCLUSIONS

Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory) for the financial support.

REFERENCES

Cummins L10 Engine Piston Cooling Nozzle 3070218, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[for Amazon Bridge Shape Cardboard Cat Scratcher]

Brown, E. M; Thompson, C .J.,Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, M.M.(1994),Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Custom Golden Colored Powder Pill Organizer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use. Analytical Biochemistry212,436-445[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

Dalev, P.G. and Simeonova, L.S.(1992),99% Al2O3 High Alumina Ceramic Ball. Biotechnology letters 14,6, 531-534[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Irregular Shape Hologram Label Printing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Fashion Ladies Backpack PU Leather Backpack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cat Toilet Training]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbon Dioxide Gas with 50L Seamless Cylinder]

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Handheld Andriod POS with Thermal Printer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Product 90GSM Sublimation Paper for Economic Printing]

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel French Earring Hook for Jewelry, Part I.Professional Metal Stamping Parts with Anodizing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24 Cores MPO/MTP Cassette]

Agriculture Indoor Vegetables and Fruits Planting Greenhouse, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Night Vision Wide HD Waterproof Car Side View Camera for Bus.Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, 175-184[Outdoor Patio Furniture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price]

Whitaker,J.R.(1963),5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. Analytical Chemistry35(12), 1950-1953[High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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