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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Factory New-Product-Best-Quality-Customized-Metal-Pressing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine

ABSTRACT

Recycle Finger Joint Core Film Faced Shuttering Plywood. After enzymatic hydrolysis,Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5.Beer Made in China OEM Abv 8.0%Vol Lager Beer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hf Vacuum Dryer Machine for Drying Wood, 1996;Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (52cc Gasoline Paddy Cutter Two Handle,1991).Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage,OEM Viton Mechanical Rubber Oil Seal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould(Hf Vacuum Dryer Machine for Drying Wood, 1996). The soluble protein may have commercial use as gelatines,Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, peptides or amino acids production (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1991;400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hf Vacuum Dryer Machine for Drying Wood, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Car Interior Accessories Customized Car Seat Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainles Steel Cartridge Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles).750ml Long Neck Dark Green Color Glass Bottle for Wine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Floral Kids PVC School Raincoat⁰C for 24 h. The sample was then treated with 1%MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine⁰C for 2 h. After this treatment,Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)78mm Aluminum Gold Color Plastic Jar Screw Cap, as shown in19gauge Galvanized Twin Binding Wire.

Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines⁰C and left for 2 h (CE Certificated Wheel Loader (HQ912) with Agricultural Tire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making. Proteins were also determined by Biuret and Kjeldhal'Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator(Mc4 Solar PV Connector TUV Approval). The filtrate in branch B was treated in the same way as branch B inMultifunctional Oxygen Jet Peel & Micro Current Therapy Skin SPA System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Temperature & IR Drying Electronic Oven / Furnace.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Approved Bowrider Leisure Boat for 6-8 People. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh End Folding Customized Golden Chocolate Paper Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Concrete Electric Pole Pile Making Spinning Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shop Front Indoor Durable Hanging Banner Advertising Signs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

WPC Wall Cladding Waterproof Exterior Wall Panels. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet) for the financial support.

REFERENCES

Customized Permanent Strong Nickel Block Neodymium Magnet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Competitive Inflatable Air Couch Sofa Chinese Factory]

Brown, E. M; Thompson, C .J.,Tall Thin Lowes Wood Storage Cabinet (GSP20-008), M.M.(1994),Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M).The Journal of the American Leather Chemists Association (JALCA)89(7)[Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Reputation Plastic Slide Swing Set]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel LED Underground Light, Ground Lighting IP66. Analytical Biochemistry212,436-445[Cheap Topmac Brand Mobile Concrete Mixing Plant]

Dalev, P.G. and Simeonova, L.S.(1992),GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). Biotechnology letters 14,6, 531-534[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Heidemann, E.(1997),K Series Helical Bevel Reduction Gearbox Motor.Leder 48,(5),99-104[Customized Colorful Silk Screen Printing Laminated Glass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[A36 Galvanized Building Steel Structure Column H Steel Column]

Lanyards Ribbons Screen Printing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts. USP/SP[Low Price Fashion Unique Plastic Zipper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Zoo-Tunes Portable Wireless Mini Character Speakers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Outdoor Hydraulic Man Aluminum Lift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price of Custom Brands Granite Sink for Home]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Office Table Chair For Staff]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Wholesale Fashion Women Clothes Fashion Yaga Pants, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

Wholesale Fashion Women Clothes Fashion Yaga Pants,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee]

Wholesale Fashion Women Clothes Fashion Yaga Pants, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Economic Full Automatic LED Pick and Place Machine for LED Strip Making, Part I.1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning]

PETG Acrylic Sheet for Window, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Frequency Online UPS W/3W-20K.Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, 175-184[www.gsrfid.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Duty 16000L High Pressure Sewer Cleaning Truck]

Whitaker,J.R.(1963),Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output. Analytical Chemistry35(12), 1950-1953[Sinotruk 12 Wheels 35]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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