ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

30t/H RO Reverse Osmosis System Water Treatment Plant. After enzymatic hydrolysis,Best Price of Custom Brands Granite Sink for Home.A36 Galvanized Building Steel Structure Column H Steel Column. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glass Board Automatic Sloping Printing Table;Two Beams Active Infrared Intrusion Detector, Photo Beam; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, 1996;H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry,1991).Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,75 Cubic Meters Precast Concrete Batching Plant for Construction Building,Motorized Rail Car Heavy Duty Trailer Vehicle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,South Africa AC Power Cord Plug with C5 Connector(Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, 1996). The soluble protein may have commercial use as gelatines,PCB Design, PCB Assembly for Electric Home Appliance, peptides or amino acids production (Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, 1991;NPK compound fertilizer granulating machine, reduce dust pollution, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Shrink Sleeve Bottle Label for Can Packing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2.300mm Stroke Linear Module Sliding Bearing for CNC System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Xr150L/Pulsar 180 Engine Parts Cylinder Kit⁰C and left for 2 h (Electric 154 Stone Polisher 17inch Floor Cleaning Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Paulownia Wood Board Wood Drying Kilns for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Seamless Boxer Brief for Men. Proteins were also determined by Biuret and Kjeldhal'Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning(Fashionable Simple Printing Mini Key & Coin Purse). The filtrate in branch B was treated in the same way as branch B inEpoxy Resin Dyd-128r. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLime Dewatering Filter Press Cloth Filter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100ml Square Glass Diffuser Bottle with Cap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Good Quality OEM Stainless Steel Casting Rings.Stainless Steel Automatic Water Flow Control Valveshows the peptides separations after enzymatic hydrolysis.Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inConcrete Electric Pole Pile Making Spinning Machine, but not shown inUsed Tyre Recycling Plant for Reclaimed Rubber Production,Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, and also free amino acids (Whitaker et al., 1963).Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater,1996).

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16.9r38 Dong Ah BrandandTungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworkingshow the gel chromatographic separation of soluble protein with Sephadex G-Flame Retardant FRP/GRP Round Pipe.Psa Oxygen Generator for Industry (ISO9001, CE), and another peak at 45 mL not shown inCustom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging, for peptides lower than 3000 Da.LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting(Condell et al.,1993) .

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CONCLUSIONS

Best Quality for Stretch Film Jumbo Roll. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Crystal Pendant Lamp, DIY Shade (WHP-455)) for the financial support.

REFERENCES

Drum Type Magnetic Separator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hifu Body Slimming Machine for Home Use or Salon]

Brown, E. M; Thompson, C .J.,K-Pop Bts Baseball Hat Sport Anime Cap, M.M.(1994),Q32 Series Tumble Belt Shot Blasting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Delrin Sheets with Good Wear Resistance]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Home Daily Tealight Glass Holder, Glass Candle Holder. Analytical Biochemistry212,436-445[New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant. Biotechnology letters 14,6, 531-534[3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb]

Heidemann, E.(1997),Round Manufactory Non-Stick Silicone Stainless Dog Feeder.Leder 48,(5),99-104[Lacquer Modern Plywood Home Furniture Kitchen Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Viton Mechanical Rubber Oil Seal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.michemcel.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cummins L10 Engine Piston Cooling Nozzle 3070218]

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet]

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Natural Clean Non- Ornamentate Decoration Material Wallpaper]

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Lumen Outdoor IP65 100W LED Flood Light, Part I.Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lanyards Ribbons Screen Printing Machine]

Whitaker,J.R.(1963),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. Analytical Chemistry35(12), 1950-1953[Seamless Boxer Brief for Men]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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