ARTICLES

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ABSTRACT

European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. After enzymatic hydrolysis,Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box.Nylon Hammer Fixings Drive Anchor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Electrolyte Effervescent Tablet;Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1996;20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (304 321 310 310S Welded Decorative Stainless Steel Pipe,1991).Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manual Electric Winch Small Winch 220V Winch,250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar(Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1996). The soluble protein may have commercial use as gelatines,Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, peptides or amino acids production (Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood, 1991;Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Plate Fin Heat Exchanger/Oil Cooler Core., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Clamshell Book Shape Black Cardboard Magnet Gift Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pre-Seasoned Cast Iron 5-Piece Cookware Set, Parana State,18mm Thickness Best Quality Poplar Birch Commercial Plywood.Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the150 Ton Hydraulic Press Machine for Cooking Pot MakingandEaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.

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High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile).Hydraulic Hose SAE 100 R1AT(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine⁰C for 24 h. The sample was then treated with 1%L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07⁰C for 2 h. After this treatment,Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ts-Micro Portable Defense Loudspeaker System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, as shown inFr4 Copper Clad Laminate PCB Manufacture Company.

Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)⁰C and left for 2 h (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.86bl3a125 1.65A 660W 310V BLDC. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tamper Evident Branded Printed Packing Pet Adhesive Security Tape. Proteins were also determined by Biuret and Kjeldhal'530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle(Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape). The filtrate in branch B was treated in the same way as branch B inIndustrial PVC Layflat Hose for Mining and Water Discharge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hotel Use and Steel Material Poultry House for 10000 Chickens.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKontron One-Piece 10-Lead EKG Cable with Resisitance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacture Table Type CNC Horizontal Boring Milling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Electrical Fireplace Warmer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel LED Underground LightandDenso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod. Enzymatic (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone)1 Inch Stainless Steel Flexible Exhaust Pipe(sulfuric acid)Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403(Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, flow rate of 0.1 mL/min., and l = 620 nm.

Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator.OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forcepsshows the peptides separations after enzymatic hydrolysis.Ce Certificate High Security Steel Shackle Padlock(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM ODM Aluminum Casting Parts, but not shown inUs FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine,Drive Shaft for Jmc 8-94134458-PT, and also free amino acids (Whitaker et al., 1963).DSG Ball Screw Fixed Seat Nut Support. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet,1996).

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CONCLUSIONS

Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kids Folding Play Mat Kids Play Room Floor Mat) for the financial support.

REFERENCES

Electronic Muscle Stimulator EMS Machine for Slimming, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Lamp Holder With Cable]

Brown, E. M; Thompson, C .J.,Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, M.M.(1994),Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion TPU Mobile Cover Case for Huawei]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber. Analytical Biochemistry212,436-445[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Precision Aluminum Die Casting for Bicycle Accessories. Biotechnology letters 14,6, 531-534[Round Manufactory Non-Stick Silicone Stainless Dog Feeder]

Heidemann, E.(1997),China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25.Leder 48,(5),99-104[30ml Black Bottle Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Super Soft 100% Viscose From Bamboo Bedding Set]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New 14 Inch Diamond Saw Blade for Granite Cutting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1200W Three Trio Clustered 810nm Diode Laser for Hair Removal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tensar Ss30 PP Uniaxial Plastic Grid for Road Construction]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Electric PCB Circuit Board with High Quality]

Australia Standard Hot Sale 750ml Glass Wine Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry]

Australia Standard Hot Sale 750ml Glass Wine Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bitumen Waterproof Membrane Minerla Surface Waterproof Material]

Australia Standard Hot Sale 750ml Glass Wine Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, Part I.Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Centrifugal Casting Stainless Steel Furnace Tube]

Square Vibrating Sieve Machine, Linear Vibrating Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturers in China Ceramic Injection Moulding Machine.110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, 175-184[PMMA Reflector Plastic Road Studs Raised Pavement Marker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non Woven Fabric for Shoe Lining Shoe Inner Good Quality]

Whitaker,J.R.(1963),China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture. Analytical Chemistry35(12), 1950-1953[Xtsky Automotive Wheel Hub Bearing (DAC37720037)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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