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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.7628 Fiberglass Cloth/Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainles Steel Cartridge Filter, 1996;750ml Long Neck Dark Green Color Glass Bottle for Wine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Floral Kids PVC School Raincoat,1991).MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock,Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,78mm Aluminum Gold Color Plastic Jar Screw Cap(Stainles Steel Cartridge Filter, 1996). The soluble protein may have commercial use as gelatines,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, peptides or amino acids production (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, 1991;0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Temperature & IR Drying Electronic Oven / Furnace.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Approved Bowrider Leisure Boat for 6-8 People. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainles Steel Cartridge Filter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High End Folding Customized Golden Chocolate Paper Box.HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fiberglass/Composite Mesh for Walkway FRP Grating, Parana State,Car Interior Accessories Customized Car Seat Cover.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainles Steel Cartridge Filter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Quality OEM Stainless Steel Casting RingsandIndoor Tub Customized Shower/Free Standing Shower Enclosure.

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Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles).Union NPT Bsp BSPT Stainless Steel Pipe Fittings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip⁰C for 24 h. The sample was then treated with 1%Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship⁰C for 2 h. After this treatment,2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tear Strip Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Healthy Grooming Kit for Caring Newborn Baby, as shown inChina Manufacturer Multi Span Polycarbonate Industrial Green House.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Aluminum Street Light Pole. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWPC Wall Cladding Waterproof Exterior Wall Panels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tall Thin Lowes Wood Storage Cabinet (GSP20-008)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel LED Underground Light, Ground Lighting IP66, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cosmetic & Food Grade Sodium Hyaluronate) for the financial support.

REFERENCES

CNC Gun Drilling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized Iron Sheet in Coil High Quality Dx51d 120 GSM Steel Gi Coil for Corrugated Roof Sheet]

Brown, E. M; Thompson, C .J.,Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, M.M.(1994),High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Durable 25kg 50kg Red PP Woven Bag Sack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EPDM Crossfit Gym Mat/Rubber Interlocking Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Jewelry Display Box for Ring Necklace Earring Storage Case. Analytical Biochemistry212,436-445[Car Seat Pockets PU Leather Car Console Side Organizer-Orange]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor Tub Customized Shower/Free Standing Shower Enclosure. Biotechnology letters 14,6, 531-534[Tamper Evident Branded Printed Packing Pet Adhesive Security Tape]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High quality Brass Forged Gate Valve with Casting Iron Handle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5tons Mini Site Dumper SD15]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.rfidfs.net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tongkai Classic Business Hardcover Leather Notebook]

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Supermarket Combined Island Chest Freezer Commercial Refrigerator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6082 and 6061 Aluminum CNC Machining Parts for Aircraft]

Supermarket Combined Island Chest Freezer Commercial Refrigerator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, Part I.Clip on Wire Shelving Data Strip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Suitable Apple iPhone and Android Charge]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods]

Whitaker,J.R.(1963),China OEM Alloy Steel Precision Casting. Analytical Chemistry35(12), 1950-1953[Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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