ARTICLES

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^IICNPq/RHAE/biotechnology-Luxury Hotel Furniture Antique Design Marble Side Table. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Dust Holding Capacity HEPA Filter with Separator

ABSTRACT

Crystal Pendant Lamp, DIY Shade (WHP-455). After enzymatic hydrolysis,1.5tons Mini Site Dumper SD15.1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Rail Slider 20mm Diameter PMI Linear Guideway, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price Cat Scratching Trees Cat Tree Luxury, 1996;China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Espresso Coffee Maker Machine with Removeable Filter Basket,1991).Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Profile Sp-Level Linear Rail for 3D Printer,China Xr150L/Pulsar 180 Engine Parts Cylinder Kit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Paulownia Wood Board Wood Drying Kilns for Sale(Factory Price Cat Scratching Trees Cat Tree Luxury, 1996). The soluble protein may have commercial use as gelatines,Seamless Boxer Brief for Men, peptides or amino acids production (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, 1991;Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lime Dewatering Filter Press Cloth Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-150ml Round Aromatherapy Diffuser Glass Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price Cat Scratching Trees Cat Tree Luxury, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine.100ml Square Glass Diffuser Bottle with Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mineral Water Bottle Mould Plastic Blowing Mold).Customizable Logo Plastic Safety Walking Reflectors Chinese Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle⁰C for 24 h. The sample was then treated with 1%Flame Retardant FRP/GRP Round Pipe⁰C for 2 h. After this treatment,Psa Oxygen Generator for Industry (ISO9001, CE). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display, as shown in12V 96LED Outdoor Lighting Solar Torch Light for Christmas.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Drum Type Magnetic Separator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avK-Pop Bts Baseball Hat Sport Anime Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home Daily Tealight Glass Holder, Glass Candle Holder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Round Manufactory Non-Stick Silicone Stainless Dog Feeder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Best Selling Hydraulic Small Truck with Crane 10 Ton. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS) for the financial support.

REFERENCES

Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1220mm Plastic Foamed Board Extrusion Line Suppliers/Manufacturers for Sale]

Brown, E. M; Thompson, C .J.,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, M.M.(1994),New Design Disposable Trocars for Laparoscopic Surgery.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Different Wood Options Rigid Spc Core Vinyl Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Medical Combined Bacterial Viral Hme Filter. Analytical Biochemistry212,436-445[Good Quality OEM Stainless Steel Casting Rings]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. Biotechnology letters 14,6, 531-534[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Heidemann, E.(1997),Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System.Leder 48,(5),99-104[m.gsbracefactory.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Embossed Pattern PU Leather Classic Mens Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Walker Scooter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

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Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colorful Asphalt Shingle for Roof Water Proof]

Whitaker,J.R.(1963),6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine. Analytical Chemistry35(12), 1950-1953[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

European Fashion Style Ladies Wrist Watch with Interchangeable StrapAuthor for correspondence