ARTICLES

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ABSTRACT

Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. After enzymatic hydrolysis,Powerful Neodymium Pot Magnet Rare Earth Pot Magnet.CNC Gun Drilling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Jewelry Display Box for Ring Necklace Earring Storage Case, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Indoor Tub Customized Shower/Free Standing Shower Enclosure, 1996;Glow in Dark Ceramic Sublimation Mug 11oz., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker,1991).Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,Supermarket Combined Island Chest Freezer Commercial Refrigerator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price(Indoor Tub Customized Shower/Free Standing Shower Enclosure, 1996). The soluble protein may have commercial use as gelatines,High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, peptides or amino acids production (Clip on Wire Shelving Data Strip, 1991;Custom Black and White Hard Laptop Case Travel Suitcase, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Concrete Road Crack-Pouring Machine for Construction., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China OEM Alloy Steel Precision Casting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Indoor Tub Customized Shower/Free Standing Shower Enclosure, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport.China Best Quality Hose Crimping Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z, Parana State,PSF 1000 Flat Plate Upper Discharge separator.Top Quality Sanitary Tube Shell Tube Heat Exchanger, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Indoor Tub Customized Shower/Free Standing Shower Enclosure, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine OpenersandPlastic Accessory for Hair Clipper.

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Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (RF Coaxail BNC Male to BNC Male Plug Connector Adapter).Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision iPhone Cases Fiber Laser Marking Machine⁰C for 24 h. The sample was then treated with 1%Auto Water Cooling Radiator Release Temperature Screw Air Compressor⁰C for 2 h. After this treatment,Injection Mold Maker for Plastic Fighter Toy. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PCBA SMT Equipment PCB Depaneling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HDPE Pipe for Water Supply (OD: 16mm-800mm), as shown in Galvanized Steel Grating. 403.

Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs⁰C and left for 2 h (110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Male & Female Stainless Steel Quick Coupling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys. Proteins were also determined by Biuret and Kjeldhal'Digital Floating Baby Care Products Frog Bath Design Thermometer( Small Harvester Model 4L-1.0). The filtrate in branch B was treated in the same way as branch B inSt20 Small Size Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Durable Inline Hockey Skate Puck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), pH 3.25., mobile phase B: buffer solution of 0.01 mol/18mm Thickness Best Quality Poplar Birch Commercial Plywood, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera) for the financial support.

REFERENCES

3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Washing Glove Nonwoven Wipes Making Machine]

Brown, E. M; Thompson, C .J.,5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, M.M.(1994),EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Clamshell Book Shape Black Cardboard Magnet Gift Box. Analytical Biochemistry212,436-445[42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor]

Dalev, P.G. and Simeonova, L.S.(1992),Fashionable Simple Printing Mini Key & Coin Purse. Biotechnology letters 14,6, 531-534[New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Anti-Slip Hot Sale Floor Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pre-Seasoned Cast Iron 5-Piece Cookware Set: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Rubber Tire Road Roller for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat Transfer Printing 3D Sublimation Heat Press Machine for T-Shirt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag]

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[12V/24V]

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4PCS High Quality Adjustable Wrench Set (FST-41)]

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Hose SAE 100 R1AT, Part I.Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service]

Whitaker,J.R.(1963),Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. Analytical Chemistry35(12), 1950-1953[High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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