ARTICLES

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ABSTRACT

Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck. After enzymatic hydrolysis,Natural Clean Non- Ornamentate Decoration Material Wallpaper.98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Super Soft 100% Viscose From Bamboo Bedding Set;Theme Jungle Plastic Stainless Steel Slide Outdoor Playground; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, 1996;110V-240V LED Filament Bulb Globe Cafe Patio String Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Sound System Vt215 Line Array Speaker,1991).Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting,Duct Evaporative Air Cooler for Warehouse Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Micro Sprinkler for Water Saving Irrigation(Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, 1996). The soluble protein may have commercial use as gelatines,Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price, peptides or amino acids production (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden, 1991;ABS Shell Plastic Injection Molding, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manual Glass Lifting Machine for Making Double Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine.Towed Bitumen Mixer Slj5-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Viscose Sodium Sulphate Anhydrous by Sateri, Parana State,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine.Orange Silica Gel 2-4 mm with High Quality, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEmpty Aluminum Cans Sleek 355ml for Beverage PackingandAroma Reed Diffuser with Ratten Sticks for Home Fragrance.

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2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803).aluminium discs for fry pan alloy 1060 1100 1050 3003(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Insulated Double Wall Bamboo Glass Water Bottle⁰C for 24 h. The sample was then treated with 1%Aroma Reed Diffuser with Ratten Sticks for Home Fragrance⁰C for 2 h. After this treatment,High Quality Fashion Ladies Backpack PU Leather Backpack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Suction Filter Cylinder Match 48 Series Filter Brands's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi, as shown inMultifunction Copper Busbar Processing for Aluminum Material.

16oz Square Shape Colors Glass Mason Jar with Embossed Design⁰C and left for 2 h ( CMC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms. Proteins were also determined by Biuret and Kjeldhal'100ml Plastic E Liquid Vape Cig Juice Dropper Bottle(High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price). The filtrate in branch B was treated in the same way as branch B inPvc Pipes Plastic Tubes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2-25mm Co-Extruded Highlight PVC Foam Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Zoo-Tunes Portable Wireless Mini Character Speakers.Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinicshows the peptides separations after enzymatic hydrolysis.CF8m Air Actuated Valve Dn15(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, but not shown in86 Type WiFi Smart Touch Switch Controll by Tuya APP,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, and also free amino acids (Whitaker et al., 1963).Premium Baby Toddler Food Feeding Silicone Bowl with Lid. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump,1996).

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Liquid Hardener Setting Cement Membrane Curing Concrete, V_e, and K_avcalculated as discussed previously.Professional Silicone Rubber Gasket for Doors Windows,Top Quality 99.95% Molybdenum Square Plates. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Design Paper Pyramid Memo Cube with Ribbon for Promotion) (Reis et al.,1989;High End L Shape Modular Sectional Sofa Patio Furniture Set Garden.,1991;110V-240V LED Filament Bulb Globe Cafe Patio String Light.,1991).HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee(1992), were lower, except for cystine and tyrosine,Flat Face Stainless Steel Female Jic Quick Release Coupling.

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CONCLUSIONS

Aluminium Glass Dropper for Glass Dropper Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card) for the financial support.

REFERENCES

L133pbd Common Rail Delphi Nozzle for Ford Mondeo, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Newest Type Smart Elevator Intercom Clear Voice Support Wired System]

Brown, E. M; Thompson, C .J.,Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, M.M.(1994),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Epoxy Resins]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Plastic Injected (injection) Parts Molding Process]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine. Analytical Biochemistry212,436-445[Commercial Coca Cola Refrigerator with Glass Door]

Dalev, P.G. and Simeonova, L.S.(1992),One Compartment Commercial Sink Without Drainboard. Biotechnology letters 14,6, 531-534[Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator]

Heidemann, E.(1997),Ge Holter Cable Snap Type Holter ECG Cable.Leder 48,(5),99-104[Automatic Fabric Laser Cutting Machine Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Blank Sublimation Mouse Pads]

304 Seamless Stainless Steel Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5.PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass. USP/SP[Screw Dosing Feeder Used for Chicken Feed Plant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-65 Polyester 35 Cotton Fabric Camouflage Printed Fabric: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Universal Manual Turret Milling Machine(Mill X6336)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2017 New Type Corrugated Cardboard Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette]

Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Cooled Heat Pump Chiller Can Be Customized]

Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polished Any Length Size Display Table Aluminium Extrusion Profile]

Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, Part I.Rolling Shutter Door Hardwares, Link Pipe, Link Clip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Condition Textured Peas Soy Protein Making Machine]

200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve.FUJI Small Home Lift for Sale, 175-184[Color Relief Glass Candy Storage Jars with Glass Lid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates]

Whitaker,J.R.(1963),Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier. Analytical Chemistry35(12), 1950-1953[Ring Die Bio Organic Fertilizer Pellet Mill Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

450mm High Quality Circular Diamond Cutting Saw Blade for Marble StoneAuthor for correspondence