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^IICNPq/RHAE/biotechnology-Plastic Medallion, Plastic Ceiling Rose. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PP PE PVC Single Wall Corrugated Pipe Making Machine Line

ABSTRACT

Lace Control Hiphugger Braless Latex Body Shaper L81187. After enzymatic hydrolysis,Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts.Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine;Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Centrifugal Casting Stainless Steel Furnace Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1996;Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Automatic Water Flow Control Valve,1991).Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet,3phase DC to AC Variable Frequency Drive Water Solar PV Inverter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Printed Cigarette Tipping Paper(Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1996). The soluble protein may have commercial use as gelatines,White Latex Paper Yellow/White Stearate Coated Abrasive Paper, peptides or amino acids production (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1991;Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Telescopic Slideway Steel Protection Accordion Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ridge Cap Panel Forming Machine Ridge Cap Making Machine.Grinding Factory Corn Maize Flour Milling Mill Making Machines-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, Parana State,PP PE PVC Single Wall Corrugated Pipe Making Machine Line.Plastic Case Navigation System Tooling Injection Mold, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor VehicleandChina Manufacture Table Type CNC Horizontal Boring Milling Machine.

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Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure Pump for Waterjet Machine).8X8mm Silver Expanded Metal Mesh in Hot Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst⁰C for 24 h. The sample was then treated with 1%100% Durable Flooring Roll for Hotel Office School and Health Project Floor⁰C for 2 h. After this treatment,Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Bottle Washing Recycling Line in China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale, as shown inHot-Sale Products GRP Tower for New Energy Industry.

Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings⁰C and left for 2 h (Healthy Grooming Kit for Caring Newborn Baby). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress. Proteins were also determined by Biuret and Kjeldhal'Sc/APC Fiber Optic Fast Hot Melt Connector(OEM Composite Machining Phenolic Titanium Parts Products Supplier). The filtrate in branch B was treated in the same way as branch B inBest Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)andWholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. Enzymatic (4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer)Food Grade PE Coated Kraft Paper for Paper Cup(sulfuric acid)Stainless Steel Single Filter Cartridge Housing for Food Grade(Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, flow rate of 0.1 mL/min., and l = 620 nm.

OEM Stainless Steel Investment Casting Bearing for Car Parts.High Frequency Online UPS W/3W-20Kshows the peptides separations after enzymatic hydrolysis.Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWater and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag, but not shown inStainless Steel Sanitary Thread Pneumatic Butterfly Valve,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, and also free amino acids (Whitaker et al., 1963).30t/H RO Reverse Osmosis System Water Treatment Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System,1996).

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Two Beams Active Infrared Intrusion Detector, Photo Beam, V_e, and K_avcalculated as discussed previously.CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand,Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Indoor Tub Customized Shower/Free Standing Shower Enclosure) (Reis et al.,1989;Manufacturer Automotive CNC Machined Parts Checking Fixture Components.,1991;Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.,1991).H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)(1992), were lower, except for cystine and tyrosine,Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry.

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CONCLUSIONS

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Household Use Food Packing 8011 Aluminum Foil) for the financial support.

REFERENCES

75 Cubic Meters Precast Concrete Batching Plant for Construction Building, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Selling Metal 5ml Perfume Spray Bottle]

Brown, E. M; Thompson, C .J.,Motorized Rail Car Heavy Duty Trailer Vehicle, M.M.(1994),South Africa AC Power Cord Plug with C5 Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PCB Design, PCB Assembly for Electric Home Appliance. Analytical Biochemistry212,436-445[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Dalev, P.G. and Simeonova, L.S.(1992),Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. Biotechnology letters 14,6, 531-534[20399560 Oil Seal 125X148X9.7 for Volvo]

Heidemann, E.(1997),NPK compound fertilizer granulating machine, reduce dust pollution.Leder 48,(5),99-104[Aluminum Stamping Part]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colorful Asphalt Shingle for Roof Water Proof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Fridge For Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),300mm Stroke Linear Module Sliding Bearing for CNC System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flat Wedge Wire Panel China Factory]

Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, Part I.Luxury Hotel Furniture Antique Design Marble Side Table. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce Approved Cheap Medical Disposable Electrical Infusion Pump]

Whitaker,J.R.(1963),1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. Analytical Chemistry35(12), 1950-1953[Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Manual 3-Way Plug ValveAuthor for correspondence