ARTICLES

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ABSTRACT

Gt4b63 Can Seamer Machine. After enzymatic hydrolysis,Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror.Glazed Metal Roof Tile Roll Forming Machine with Ce. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing, 1996;Extruder Floating/Sinking Fish Feed Pellet Dryer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker,1991).High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V,Quality Assured Magnetic Building Blocks for Children,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System(Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing, 1996). The soluble protein may have commercial use as gelatines,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, peptides or amino acids production (Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine, 1991;Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cummins L10 Engine Piston Cooling Nozzle 3070218., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use.99% Al2O3 High Alumina Ceramic Ball-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Price Long Life Portable Peltier Air Cooler(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Handheld Andriod POS with Thermal Printer, Parana State,RF Coaxail BNC Male to BNC Male Plug Connector Adapter.Stainless Steel French Earring Hook for Jewelry, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Speed Automatic Slitting Machine for Plastic FilmandCe Approved Bowrider Leisure Boat for 6-8 People.

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Professional Metal Stamping Parts with Anodizing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (RV UPS Power 48V 80ah LiFePO4 Battery Pack).Agriculture Indoor Vegetables and Fruits Planting Greenhouse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Night Vision Wide HD Waterproof Car Side View Camera for Bus⁰C for 24 h. The sample was then treated with 1%Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade⁰C for 2 h. After this treatment,5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)E2000/APC Fibre Optic Leads and Cables's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable, as shown inMade in China Aluminum Extrusion Window and Door Profile.

Physical Foaming Wire Extruder⁰C and left for 2 h (20 Years Factory! Double Channel Surgical Syringe Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine. Proteins were also determined by Biuret and Kjeldhal'Plastic PVC 3 Way Elbow Pipe Fittings(Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine). The filtrate in branch B was treated in the same way as branch B inSplicing Solution Mechanical Coupling Rebar Sleeve for Reinforcement. The final soluble protein shown were used for peptides separations using gel permeation chromatography,D405 Trio 300W 4 Voice Coil Selemium Driver De-1001.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%150 micron steel mesh filter magnetic hydraulic filter cartridge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gold Parquet Marble Copper Side End Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Oxalic Acid for 99.6% Purity.ANSI B16.5 Stainless Steel SS316 Forging Blind Flangeshows the peptides separations after enzymatic hydrolysis.Kids Waterproof Softshell Jacket with Brushed Fleece Inside(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL, but not shown inCleaning Products Sponge Vacuumized Pack 65mm,CNC Turned Aluminum Anodized Auto Part, and also free amino acids (Whitaker et al., 1963).4-20mA PT100 Temperature Transmitter (PCT202). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing,1996).

Flour

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Easy Soak off OEM 10ml Gel Nail Polish, V_e, and K_avcalculated as discussed previously.Cosmetic Case Box Packaging Makeup Case for Outdoor,Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mineral Water Bottle Mould Plastic Blowing Mold) (Reis et al.,1989;Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine.,1991;Extruder Floating/Sinking Fish Feed Pellet Dryer.,1991).Aluminum Foil Container Making Machine, Pneumatic Punching Machine(1992), were lower, except for cystine and tyrosine,LED Pixel Light U4 1.2W.

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CONCLUSIONS

Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover) for the financial support.

REFERENCES

15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Round Hollywood Vanity Mirror]

Brown, E. M; Thompson, C .J.,10/16A EV Potable Charging Station Type 2 621962 Plug Cee, M.M.(1994),Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794.The Journal of the American Leather Chemists Association (JALCA)89(7)[38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacture Table Type CNC Horizontal Boring Milling Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock. Analytical Biochemistry212,436-445[Best Price 201 304 Stainless Steel Coils]

Dalev, P.G. and Simeonova, L.S.(1992),Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED. Biotechnology letters 14,6, 531-534[High Pressure Pump for Waterjet Machine]

Heidemann, E.(1997),Low Noise Ventilating Blower Fan for Agriculture.Leder 48,(5),99-104[Top Hot Rolled Ms Mild Steel Checkered Sheet/Plate Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot-Sale Products GRP Tower for New Energy Industry]

IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Waterproof Chipboard, Melamine Chipboard for Furniture.Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women. USP/SP[Healthy Grooming Kit for Caring Newborn Baby]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Composite Machining Phenolic Titanium Parts Products Supplier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory made hot-sale Ac1416 Buffer - AC0806 - Zhuohui]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Frequency Online UPS W/3W-20K]

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Foshan Terracotta Facade Panel for Commercial Building Curtain Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, Part I.Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box.Factory Truck/Bus Spray Paint Booth /Industry Paint Booth, 175-184[Brick Machine Pallet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Whitaker,J.R.(1963),Enamelled Heavy Duty Cast Iron Skillet. Analytical Chemistry35(12), 1950-1953[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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