ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Digital Floating Baby Care Products Frog Bath Design Thermometer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine

ABSTRACT

Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. After enzymatic hydrolysis,FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters.Colorful Asphalt Shingle for Roof Water Proof. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.18mm Thickness Best Quality Poplar Birch Commercial Plywood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996;Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nylon Hammer Fixings Drive Anchor,1991).OEM Electrolyte Effervescent Tablet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet(European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996). The soluble protein may have commercial use as gelatines,20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, peptides or amino acids production (304 321 310 310S Welded Decorative Stainless Steel Pipe, 1991;Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manual Electric Winch Small Winch 220V Winch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashionable Simple Printing Mini Key & Coin Purse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Computer Screen etc).CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kids Folding Play Mat Kids Play Room Floor Mat⁰C for 24 h. The sample was then treated with 1%Roller Window Shutter for Residential Usage⁰C for 2 h. After this treatment,Pre-Seasoned Cast Iron 5-Piece Cookware Set. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, as shown inFst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output.

Hydraulic Hose SAE 100 R1AT⁰C and left for 2 h (Three F Female Connector Adapter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Proteins were also determined by Biuret and Kjeldhal'Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder). The filtrate in branch B was treated in the same way as branch B inAlloy Material Aluminum Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ts-Micro Portable Defense Loudspeaker System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Supply for Laparoscopic Instrument (MIM Straight Scissors)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tamper Evident Branded Printed Packing Pet Adhesive Security Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hotel Use and Steel Material Poultry House for 10000 Chickens, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Recycle Finger Joint Core Film Faced Shuttering Plywoodand50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. Enzymatic (Kontron One-Piece 10-Lead EKG Cable with Resisitance)Best Price of Peanut Digger / Groundnut Harvester in Australian Africa(sulfuric acid)China Manufacture Table Type CNC Horizontal Boring Milling Machine(European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%White Electrical Fireplace Warmer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%White Electrical Fireplace Warmer, flow rate of 0.1 mL/min., and l = 620 nm.

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor.Hydraulic Press Corrugated Cold Roll Automatically Forming Machineshows the peptides separations after enzymatic hydrolysis.Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, but not shown inEVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, and also free amino acids (Whitaker et al., 1963).1 Inch Stainless Steel Flexible Exhaust Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag,1996).

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Ce Certificate High Security Steel Shackle Padlock, V_e, and K_avcalculated as discussed previously.Drive Shaft for Jmc 8-94134458-PT,DSG Ball Screw Fixed Seat Nut Support. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED Pixel Light U4 1.2W) (Reis et al.,1989;304 321 310 310S Welded Decorative Stainless Steel Pipe.,1991;Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box.,1991).Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car(1992), were lower, except for cystine and tyrosine,Apex Digital UV Printers for Aluminum Board Printing UV6090.

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CONCLUSIONS

24 Molds Commercial Ice Cream Cone Maker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet) for the financial support.

REFERENCES

Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Direct Custom Wholesale Cheap Embroidery Woven Patches Iron-on Clothing]

Brown, E. M; Thompson, C .J.,Fashion Children Fur Pompom Beanie Scarf Knit Set, M.M.(1994),Steel Jacket Steam Insulation Pipeline Production Best Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Clear Customized PVC Cosmetic Package Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Haa 800mAh NiMH Battery Pack. Analytical Biochemistry212,436-445[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Dalev, P.G. and Simeonova, L.S.(1992),Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer. Biotechnology letters 14,6, 531-534[High Speed Mixer for Plastic Extrusion Line]

Heidemann, E.(1997),Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts.Leder 48,(5),99-104[Aluminum-Steel Clad Induction Plate Used for Rail Transportation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cosmetic & Food Grade Sodium Hyaluronate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Handheld Andriod POS with Thermal Printer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Inflatable Water Sports Equipment Products Machine Aqua Bike Water Bike]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Precision Aluminum Die Casting for Bicycle Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric DC 24V Geared Motor for Auto Doors]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25, Part I.Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1200W Three Trio Clustered 810nm Diode Laser for Hair Removal.Australia Standard Hot Sale 750ml Glass Wine Bottle, 175-184[Complete Wood Paint Production Line Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve]

Whitaker,J.R.(1963),Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay. Analytical Chemistry35(12), 1950-1953[Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal VaricesAuthor for correspondence