ARTICLES

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ABSTRACT

Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. After enzymatic hydrolysis,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase.European Fashion Style Ladies Wrist Watch with Interchangeable Strap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter;Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Combination Weighing Packing Machine Jy-420A, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate, 1996;Tfl Blue Rubber Transmission Belts/Automotive Spare Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China,1991).Flat Wedge Wire Panel China Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625,Best Selling Hydraulic Small Truck with Crane 10 Ton,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector(Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate, 1996). The soluble protein may have commercial use as gelatines,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, peptides or amino acids production (New Design Disposable Trocars for Laparoscopic Surgery, 1991;Disposable Medical Combined Bacterial Viral Hme Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Pineapple Canning Plant.Embossed Pattern PU Leather Classic Mens Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile).Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cookies Cake Automatic Rotary Packing Machine⁰C for 24 h. The sample was then treated with 1%Building Material Quick Install Types of Scaffolding Couplers⁰C for 2 h. After this treatment,Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, as shown inLarge Capacity Food Processing Cooling Tower Conveyor Equipment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Topmac Brand Mobile Concrete Mixing Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColor Stretch Film for Pallet Wrap Use(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hemp Recycle Polyester Blenched Jersey Knit Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices.12V 96LED Outdoor Lighting Solar Torch Light for Christmasshows the peptides separations after enzymatic hydrolysis.Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Items Gh/Genatropin with Naked Vial, but not shown in38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, and also free amino acids (Whitaker et al., 1963).High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate,1996).

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CONCLUSIONS

Toughened Railing Glass. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger) for the financial support.

REFERENCES

Delrin Sheets with Good Wear Resistance, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[LED Pick and Place Machine LED640V]

Brown, E. M; Thompson, C .J.,High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery, M.M.(1994),Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service.The Journal of the American Leather Chemists Association (JALCA)89(7)[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies. Analytical Biochemistry212,436-445[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Gas and Water End Protective Covers (YZF-H26). Biotechnology letters 14,6, 531-534[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Heidemann, E.(1997),400W Emergency Backup Battery Powerstation Portable Solar Generator.Leder 48,(5),99-104[Full Color Custom T Shirts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof.Automatic Electric Hydraulic Metal Bending Machine. USP/SP[Different Wood Options Rigid Spc Core Vinyl Flooring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quality OEM Stainless Steel Casting Rings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6082 and 6061 Aluminum CNC Machining Parts for Aircraft: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Concrete Water Reducing Admixture Liquid Polycarboxylate-Based Water Reducer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

1054 FRP Pressure Vessel for Water Softener & Water Treatment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

1054 FRP Pressure Vessel for Water Softener & Water Treatment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Manufacturer Multi Span Polycarbonate Industrial Green House]

1054 FRP Pressure Vessel for Water Softener & Water Treatment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, Part I.Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

HDPE Large Diameter Hollowness Wall Winding Pipe Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Non-Woven Medical Outside Earloop Mask Making Machine.Professional Family Nonstick Cast Iron Frying Pan, 175-184[Mini Kerosene Car Fridge for Hotel Yacht Auto]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Whitaker,J.R.(1963),Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. Analytical Chemistry35(12), 1950-1953[20399560 Oil Seal 125X148X9.7 for Volvo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEMAuthor for correspondence