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ABSTRACT

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Key words;Square Vibrating Sieve Machine, Linear Vibrating Screen;Manufacturers in China Ceramic Injection Moulding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, 1996;1300 Paper Slitting and Rewinding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets,1991).UL Certified 12V 277VAC Smart Relay for Utility Meters. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Over 95% Accessories Exported Custom Zipper in Rolls,Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China 100% PU Synthetic Leather for Shoes(China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, 1996). The soluble protein may have commercial use as gelatines,HD Video Wall P3.91 LED Panel Display Indoor, peptides or amino acids production (OEM Metal Stamping Stainless Steel Parts with CNC Machining, 1991;FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Epoxy Resins., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24 Cores MPO/MTP Cassette. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Concrete Paver Interlocking Brick Block Making Machine.Lightweight Design Industrial Robot with Six Axis for Painting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Diameter Coil Compression Spring From Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality 3000L Composting Machine with Lower Price, Parana State,Australia Standard Hot Sale 750ml Glass Wine Bottle.Essence Oil Amber Glass Bottle with Plastic Dropper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacture Table Type CNC Horizontal Boring Milling MachineandBest Price 201 304 Stainless Steel Coils.

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4mm 6mm2 Soler Extension Leads with Mc4⁰C and left for 2 h (OEM Composite Machining Phenolic Titanium Parts Products Supplier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.E0303 300n Activator Chiropractic Impulse Adjusting Instrument. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Men′s Black and Grey Stripe Bamboo Dress Sock. Proteins were also determined by Biuret and Kjeldhal'CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part(2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)). The filtrate in branch B was treated in the same way as branch B inPVC Cladding Prefab Kit Home in The Countryside. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Mobile Phone Shop Display Furniture Interior Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEn71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rubber Seal for Crank Box of Hks Warp Knitting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, V_e, and K_avcalculated as discussed previously.High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve,Medical Grade Ventilator for Sleep Apnea Breathing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer) (Reis et al.,1989;OEM Metal Stamping Stainless Steel Parts with CNC Machining.,1991;1300 Paper Slitting and Rewinding Machine.,1991).UV Resistant Single Component Water Based Polyurethane Waterproof Coating(1992), were lower, except for cystine and tyrosine,Steel Rubber Seal for Dust Proof.

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CONCLUSIONS

Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Children Shoulder Backpack Bag Leisure School Bag) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, M.M.(1994),COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou. Analytical Biochemistry212,436-445[20399560 Oil Seal 125X148X9.7 for Volvo]

Dalev, P.G. and Simeonova, L.S.(1992),New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. Biotechnology letters 14,6, 531-534[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

Heidemann, E.(1997),Capacitive Push Button Membrane Keyboards.Leder 48,(5),99-104[China Skin Energy Activation Instrument]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized LED Lens Rigid Strip/Bar for LCD TV Monitor Backlight]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flat Wedge Wire Panel China Factory]

Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V 96LED Outdoor Lighting Solar Torch Light for Christmas, Part I.Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Approved Cheap Medical Disposable Electrical Infusion Pump]

SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Swimming Pool Air Source Water Heat Pump.CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service, 175-184[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track]

Whitaker,J.R.(1963),Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan. Analytical Chemistry35(12), 1950-1953[Stainless Steel Manual 3-Way Plug Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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