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ABSTRACT

Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. After enzymatic hydrolysis,Composite Plate Dedicated Drop Hose Gear Metering Pump Price.Brake Fluid Tank Container Hot Plate Plastic Welding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices;Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, 1996;Puffed Core Filling Sanck Production Machine with Ce From China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting,1991).Cummins Fuel Injector Kit (3076736 3095023). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5,Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Therm Forming Filling Sealing Machine, Lid Press Machine(High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Electric 100W Three Wheels Toy Car for Sale, peptides or amino acids production (New Design Customized 100% Cotton Home Towel Bath Towel, 1991;Toughened Railing Glass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Delrin Sheets with Good Wear Resistance., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies.Plastic Gas and Water End Protective Covers (YZF-H26)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:400W Emergency Backup Battery Powerstation Portable Solar Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, Parana State,HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCable Railng Spiral Staircase with PVC Handrail for Interior Space SavingandEco-Friendly Vegetable Oil Cast Iron Multi Frying Pan.

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Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch).HDPE Large Diameter Hollowness Wall Winding Pipe Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Non-Woven Medical Outside Earloop Mask Making Machine⁰C for 24 h. The sample was then treated with 1%Professional Family Nonstick Cast Iron Frying Pan⁰C for 2 h. After this treatment,Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashionable Clothing Garment Paper Swing Tags (ST-01)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Horizontal Directional Drilling Machine SHD28, as shown inViscose Sodium Sulphate Anhydrous by Sateri.

Electrial Galvanized Conduit Box/Gi Pipe Fitting⁰C and left for 2 h (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High quality Brass Forged Gate Valve with Casting Iron Handle. Proteins were also determined by Biuret and Kjeldhal'Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)(Electronic Muscle Stimulator EMS Machine for Slimming). The filtrate in branch B was treated in the same way as branch B inElectric Cable Tightener. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cog Mono Round Display LCD Module. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTV Stand Carving European LED Lights Heating Fireplace (320SS)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Low Price Fashion Unique Plastic Zipper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silica Gel- Indicating Blue to Pink After Adsorption, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Offer High Quality Square and Round Cast Iron Manhole Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Gas Spring Strut Gas Shock for Wall Bed. 15mmshows the peptides separations after enzymatic hydrolysis.Industrial Bedsheet Folding Machine (ZD-3000)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inClutch Brake Booster for Yutong Zk6127h Bus, but not shown inHorizontal Directional Drilling Machine SHD28,Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, and also free amino acids (Whitaker et al., 1963).OEM ODM Aluminum Casting Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts,1996).

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CONCLUSIONS

RF Shielding MRI Door with Brass Frame for RF Cage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6082 and 6061 Aluminum CNC Machining Parts for Aircraft) for the financial support.

REFERENCES

Geotextile Beg Used for Beach Restoration Geobag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Zipper Flat Bottom Coffee Bag One Way Valve Manufacturer in China]

Brown, E. M; Thompson, C .J.,Reusable Silicone Food Storage Bag, M.M.(1994),Dental Emax Empress Crown From China Dental Lab.The Journal of the American Leather Chemists Association (JALCA)89(7)[Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price of Peanut Digger / Groundnut Harvester in Australian Africa]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt. Analytical Biochemistry212,436-445[A616 Axe with Wooden Handle Series]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. Biotechnology letters 14,6, 531-534[Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood]

Heidemann, E.(1997),Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill.Leder 48,(5),99-104[Secondary Hot Rolled Steel Coil 201 Baby Stainless Steel Coil for Tubing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine]

Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dubai Products Rectified Tile for Wall.ANSI B16.5 Stainless Steel SS316 Forging Blind Flange. USP/SP[Factory Wholesale Reach Standard Home and Office Furniture Fitting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Silver Anodized Aluminium 135 Degree Angle Bracket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Tubeless Industrial Wheel 16.5X9.75 for Skid Steer and Forklift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Integrated Hydraulic Steering Control Units (BZZ1F SCU)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Drum Rotator]

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Lid Press Machine]

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Model Water Source Heat Pump (SLW100D), Part I.Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets]

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets.Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, 175-184[Qt 4-20 C Brick Making Machine Price List / Block Machine Factory/ Block Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Unfolding Grocery Push Carts]

Whitaker,J.R.(1963),High Capacity Vibrating Screen Manufacturer. Analytical Chemistry35(12), 1950-1953[Bwb-306 Custom Printing Outdoor Sports Wristband]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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