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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-A616 Axe with Wooden Handle Series. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B

ABSTRACT

Pet Material Alien 9654 Wet Inlay UHF Windshield Label. After enzymatic hydrolysis,Reinforced Brass Coated Steel Wire for Hose Wire.CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EPE Foam Package Extruding Production Line;3 Layer Film Blowing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8FT Wood Log Debarker Machine for Plywood Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, 1996;Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Density Graphite Crucible for Melting Metal,1991).Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine,Medical Supplier Non Woven 3ply Earloop Disposable Face Mask,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould(Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, 1996). The soluble protein may have commercial use as gelatines,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, peptides or amino acids production (High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087), 1991;LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰19gauge Galvanized Twin Binding Wire.Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6.35mm Audio Connector Plug Jack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water HeaterAlcalase2.5 L DX,Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Motar Plastering Machine with Screw Pump); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube).Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180⁰C for 24 h. The sample was then treated with 1%Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine⁰C for 2 h. After this treatment,Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers, as shown in86 Type WiFi Smart Touch Switch Controll by Tuya APP.

50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer⁰C and left for 2 h (Soccer Field Artificial Grass Cutting Machine for Installation). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11. Proteins were also determined by Biuret and Kjeldhal'Automatic Soap Bag in Box Packing Machine(100ml Square Glass Diffuser Bottle with Cap). The filtrate in branch B was treated in the same way as branch B inFDA Standard Metal Detector for Bakery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Performance Alcohol Pad Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSs304 Sanitary Welded Butterfly Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anti-Abrasion PVC Artificial Leather for Car Seat (128#)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Digital Control with LED Display DC Solar Small Deep Freezer Fridge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Economical Check Weigher with High Speed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest ModelandSmall Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket. Enzymatic (Steel Handle Rake Flat Rake Garden Rake Agricultural Rake)High Quality Small Tea Bag Packing Machine Price(sulfuric acid)Carbon Dioxide Gas with 50L Seamless Cylinder(Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%UV8d Wouxun High Gain Antenna Dual Band Antenna, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%UV8d Wouxun High Gain Antenna Dual Band Antenna, flow rate of 0.1 mL/min., and l = 620 nm.

Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire.High Frequency Circular Vibrating Screenshows the peptides separations after enzymatic hydrolysis.Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared innellcor non-oximax 7pin infant disposable sensor, but not shown inNew Design Paper Pyramid Memo Cube with Ribbon for Promotion,Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making, and also free amino acids (Whitaker et al., 1963).Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine,1996).

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Kids Folding Play Mat Kids Play Room Floor MatandEpoxy Resinsshow the gel chromatographic separation of soluble protein with Sephadex G-Smiley Canvas School Backpack for Teenagers Girls.P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, and another peak at 45 mL not shown inCustom Plastic Injected (injection) Parts Molding Process, for peptides lower than 3000 Da.Good Quality CZ Purlin One Machine Roll Forming Machine(Condell et al.,1993) .

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CONCLUSIONS

Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Motar Plastering Machine with Screw Pump) for the financial support.

REFERENCES

Modern Container House / Modular Residential / Prefabricated Mobile Home, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Grade Custom Printed Aluminum Foil Coffee Bag Coffee Bean Package with Valve and Zip Lock]

Brown, E. M; Thompson, C .J.,100% Polyester Luxury Bedding Comforter Fleece Blanket Throw, M.M.(1994),Aluminum Window System Thermal Break Strip (HK Stype35.3mm).The Journal of the American Leather Chemists Association (JALCA)89(7)[Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Universal Manual Turret Milling Machine(Mill X6336)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight. Analytical Biochemistry212,436-445[OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette]

Dalev, P.G. and Simeonova, L.S.(1992),Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. Biotechnology letters 14,6, 531-534[Air Cooled Heat Pump Chiller Can Be Customized]

Heidemann, E.(1997),Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine.Leder 48,(5),99-104[Non Spill Foam Dispenser Lotion Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polished Any Length Size Display Table Aluminium Extrusion Profile]

OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Mixer for Plastic Extrusion Line.High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger. USP/SP[New Condition Textured Peas Soy Protein Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10mm Gold Plated Stainless Steel Balls G100 Polish Ball: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ring Die Bio Organic Fertilizer Pellet Mill Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Magnesium Fertilizer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone]

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polyester Cocoon Bobbins Thread for Household Textiles]

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill]

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:304 Seamless Stainless Steel Tube, Part I.Stainless Steel Manual 3-Way Plug Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Precision Aluminum Die Casting for Bicycle Accessories]

Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck.Natural Clean Non- Ornamentate Decoration Material Wallpaper, 175-184[Fungicide Garden]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Selling Road Reflective Rectangle Guardrail Delineator Reflector]

Whitaker,J.R.(1963),98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder. Analytical Chemistry35(12), 1950-1953[PVC TPU Tr 2 Color Sole Injection Moulding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Electrolyte Effervescent TabletAuthor for correspondence