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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toughened Railing Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Delrin Sheets with Good Wear Resistance, 1996;High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service,1991).Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Gas and Water End Protective Covers (YZF-H26),400W Emergency Backup Battery Powerstation Portable Solar Generator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof(Delrin Sheets with Good Wear Resistance, 1996). The soluble protein may have commercial use as gelatines,Automatic Electric Hydraulic Metal Bending Machine, peptides or amino acids production (6082 and 6061 Aluminum CNC Machining Parts for Aircraft, 1991;1054 FRP Pressure Vessel for Water Softener & Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non Woven Fabric for Shoe Lining Shoe Inner Good Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Delrin Sheets with Good Wear Resistance, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.Non-Woven Medical Outside Earloop Mask Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Family Nonstick Cast Iron Frying Pan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Offer High Quality Square and Round Cast Iron Manhole Cover⁰C and left for 2 h (Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Tight Long Sleeves off Shoulders Celebration Bandage Dress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors. Proteins were also determined by Biuret and Kjeldhal'4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine(Anti-Theft EAS Hard Tag Tag). The filtrate in branch B was treated in the same way as branch B inNIBP. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Road Safety Reflective Waterproof Plastic Reflectors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Gas Spring Strut Gas Shock for Wall Bed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM ODM Aluminum Casting Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Pressure Low Volume 2900rpm electric Water Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Waterproof Folding Marquee Canopy Quick up TentandNew Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Enzymatic (Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240))Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch(sulfuric acid)Multifunction Copper Busbar Processing for Aluminum Material(Delrin Sheets with Good Wear Resistance, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Men Genuine Leather Sling Shoulder Satchel Messenger Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Men Genuine Leather Sling Shoulder Satchel Messenger Bag, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Horizontal Directional Drilling Machine SHD28) for the financial support.

REFERENCES

Integrated Hydraulic Steering Control Units (BZZ1F SCU), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Back White PU Chair Home Furniture Dining Chair Silver Hotel Chairs]

Brown, E. M; Thompson, C .J.,New Model Water Source Heat Pump (SLW100D), M.M.(1994),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Outdoor Hydraulic Man Aluminum Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[16oz Square Shape Colors Glass Mason Jar with Embossed Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube. Analytical Biochemistry212,436-445[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

Dalev, P.G. and Simeonova, L.S.(1992),10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. Biotechnology letters 14,6, 531-534[Private Customized High Quality Printed Shipping Labels]

Heidemann, E.(1997),Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.Leder 48,(5),99-104[Nylon Hammer drive Anchor with Zinc]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality CZ Purlin One Machine Roll Forming Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Premium Baby Toddler Food Feeding Silicone Bowl with Lid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Density Graphite Crucible for Melting Metal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Assembly Jigs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Long Stroke Hydraulic Cylinder for Constraction Machine]

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper]

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst]

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts, Part I.Cheese Puffed Snacks Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25]

Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China High Profit Automatic Pet Extruder Machine.Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), 175-184[Ski Snow Jackets For Men]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325]

Whitaker,J.R.(1963),Best Price Spc Lvt PVC Vinyl Flooring for Home Office. Analytical Chemistry35(12), 1950-1953[Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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