ARTICLES

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ABSTRACT

Factory Price Cat Scratching Trees Cat Tree Luxury. After enzymatic hydrolysis,China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Espresso Coffee Maker Machine with Removeable Filter Basket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Paulownia Wood Board Wood Drying Kilns for Sale, 1996;Seamless Boxer Brief for Men., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning,1991).Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies,Lime Dewatering Filter Press Cloth Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,150ml Round Aromatherapy Diffuser Glass Bottle(High Quality Paulownia Wood Board Wood Drying Kilns for Sale, 1996). The soluble protein may have commercial use as gelatines,C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, peptides or amino acids production (100ml Square Glass Diffuser Bottle with Cap, 1991;Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Paulownia Wood Board Wood Drying Kilns for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable CAT6 UTP RJ45 8P8C Modular Plug.3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality OEM Stainless Steel Casting Rings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear).Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom small plastic printed box for keychain (PET box)⁰C for 24 h. The sample was then treated with 1%Fence Mesh Welding Machine⁰C for 2 h. After this treatment,20 Years Factory! Double Channel Surgical Syringe Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Quality for Stretch Film Jumbo Roll's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Drum Type Magnetic Separator, as shown inUniversal Manual Turret Milling Machine(Mill X6336).

K-Pop Bts Baseball Hat Sport Anime Cap⁰C and left for 2 h (OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Q32 Series Tumble Belt Shot Blasting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Daily Tealight Glass Holder, Glass Candle Holder. Proteins were also determined by Biuret and Kjeldhal'Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant(Air Cooled Heat Pump Chiller Can Be Customized). The filtrate in branch B was treated in the same way as branch B inDoor Flood Barrier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Round Manufactory Non-Stick Silicone Stainless Dog Feeder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Lumen Outdoor IP65 100W LED Flood Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle) for the financial support.

REFERENCES

3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Home Appliance Membrane Panels/Lenses with SGS]

Brown, E. M; Thompson, C .J.,High Efficiency Pineapple Canning Plant, M.M.(1994),Embossed Pattern PU Leather Classic Mens Wallet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Container House / Modular Residential / Prefabricated Mobile Home]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[European Standards 100A 24V Electrical Relay for Green Energy]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles. Analytical Biochemistry212,436-445[Portable Ink Date Printing Machine for Plastic Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Casting PE Film with Ash for Adult Diaper Back Sheet. Biotechnology letters 14,6, 531-534[Kids Waterproof Softshell Jacket with Brushed Fleece Inside]

Heidemann, E.(1997),Supra Brand Laundry Liquid Baby Formula.Leder 48,(5),99-104[Rental Led Video Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger.Cleaning Products Sponge Vacuumized Pack 65mm. USP/SP[150ml Round Aromatherapy Diffuser Glass Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mineral Water Bottle Mould Plastic Blowing Mold: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Samsung Excavator Parts Tcm Oil Seal Travel Motor Seal Kit Oil Seal Removal Tool Roten Mechanical Seal Excavator Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[EVA Puzzle Mat with Letters for Kids Playing Customized Pictures]

High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Electrical Fireplace Warmer]

High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts]

High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, Part I.Cookies Cake Automatic Rotary Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

Building Material Quick Install Types of Scaffolding Couplers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury.Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, 175-184[Honeywell Gas Detector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Whitaker,J.R.(1963),Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft. Analytical Chemistry35(12), 1950-1953[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Experienced Construction Steel Structure Building DesignAuthor for correspondence