ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Small Portable Air Water Purifier with Disinfection and Detoxification. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX

ABSTRACT

1850mm*80mm Stable Power Laser Tube. After enzymatic hydrolysis,White Color Premium Quality Disposable Plastic PVC Apron.Galvanized Bar Grating. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ring Die Bio Organic Fertilizer Pellet Mill Machine;Hot Dipped Galvanized Deer Fence From Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996;Cheap Customized Aluminium Sand Casting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper,1991).Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China,New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality RV Electric Seating Recliner(Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996). The soluble protein may have commercial use as gelatines,2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, peptides or amino acids production (100% Hand-Painted Canvas Oil Painting Wall Art for Trees, 1991;Factort Price Cattle Beef Slaughtering Equipment for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Supreme 524 Mv Sea Kayak., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Definition Outdoor Video Advertising LED Billboard.Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 Stainless Steel Expansion Anchor Bolt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Composite Machining Phenolic Titanium Parts Products Supplier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lab Water Instruments/Lab Water Purification System Micro).DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing⁰C for 24 h. The sample was then treated with 1%Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail⁰C for 2 h. After this treatment,Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Xtsky Automotive Wheel Hub Bearing (DAC37720037), as shown inProper Price Good Quality 10ml 20ml Airless Pump Bottle.

Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle⁰C and left for 2 h (Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FTTH Optical Network Terminal for Gpon ONU and CATV. Proteins were also determined by Biuret and Kjeldhal'OEM Customized Polyester Cotton National President Election Supporters Printed Scarf(Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600). The filtrate in branch B was treated in the same way as branch B inAluminum Alloy LED Torch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,D8tc High Performance 3-Electrode Motorcycle Spark Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.19m Mobile LED Display Billboard for Advertising Screens⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nice Appearance Brass Metal Sheet Stamping, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Starter Motor Testing Equipment for Truck, Bus, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl.Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouseshows the peptides separations after enzymatic hydrolysis.Hot Sale Flat New Design Ladies Winter Warm Shoes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp, but not shown inWinter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine,Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, and also free amino acids (Whitaker et al., 1963).OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility,1996).

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CONCLUSIONS

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Bgf-800 Pet Dry Laminating Machine, M.M.(1994),Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse.The Journal of the American Leather Chemists Association (JALCA)89(7)[Extruded Polystyrene Foam Board Custom Edges S/L]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ High Pressure Lay Flat Hose for Industrial Discharge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging. Analytical Biochemistry212,436-445[10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating. Biotechnology letters 14,6, 531-534[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Heidemann, E.(1997),New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine.Leder 48,(5),99-104[Hot-Rolled Dual-Sided Stainless Clad Plate 4 (1 2 1) mm Thick]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel French Earring Hook for Jewelry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HAIRUI 850A+ 600W SMD Rework Soldering Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.hansymlaser.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

High Quality Oval Shape Cast Iron Dutch Oven, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box]

High Quality Oval Shape Cast Iron Dutch Oven,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109]

High Quality Oval Shape Cast Iron Dutch Oven, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, Part I.Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FTTH Optical Network Terminal for Gpon ONU and CATV]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.High Performance Fresh Air Handling Unit for Marine, 175-184[Shenzhen Express Turnkey PCB Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Automotive Heater Control Valve]

Whitaker,J.R.(1963),Community IC Access Card Induction ID Time Card Smart Card. Analytical Chemistry35(12), 1950-1953[Stainless Steel Single Filter Cartridge Housing for Food Grade]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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