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ABSTRACT

Irregular Shape Hologram Label Printing. After enzymatic hydrolysis,High Speed Rope Weaving Machine.Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996;China Durable 25kg 50kg Red PP Woven Bag Sack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock,1991).Mini Recorder Sound Box for Musical Box & Plush Toys. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fake Fish Luminescent Tropical Fish Fish Tank Decoration,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure Die Casting Aluminum Die Casting Gear Box Housing(Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996). The soluble protein may have commercial use as gelatines,Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, peptides or amino acids production (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1991;CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Portable Air Water Purifier with Disinfection and Detoxification., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1850mm*80mm Stable Power Laser Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White Color Premium Quality Disposable Plastic PVC Apron.Galvanized Bar Grating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ring Die Bio Organic Fertilizer Pellet Mill Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material).New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality RV Electric Seating Recliner⁰C for 24 h. The sample was then treated with 1%2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc⁰C for 2 h. After this treatment,100% Hand-Painted Canvas Oil Painting Wall Art for Trees. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factort Price Cattle Beef Slaughtering Equipment for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Supreme 524 Mv Sea Kayak, as shown inCosmetic & Personal Care Grade Xanthan Gum.

API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve⁰C and left for 2 h (CNC Machinery Aluminum and ABS Mock up Prototypes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Definition Outdoor Video Advertising LED Billboard. Proteins were also determined by Biuret and Kjeldhal'Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm(Thick Wall Large Diameter Aluminium Tube Manufacture). The filtrate in branch B was treated in the same way as branch B inBanner Advertising 510g 1000*1000 Banner Coated Compare Price Hot Laminated Banner Coated for Light Box Poster. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 Stainless Steel Expansion Anchor Bolt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Small Plug-in Insect Killer Mosquito Killing Lamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Customize PU Leather Hardcover Notebook with Window(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sale Starter Motor Testing Equipment for Truck, Bus. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility) for the financial support.

REFERENCES

Home Machine 2019 New Commercial Treadmill Gym Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Swimming Pool Equipment Stainless Steel 2 Steps 3 Steps 4 Steps 5 Steps Pool Ladder]

Brown, E. M; Thompson, C .J.,New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.M.(1994),Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[1850mm*80mm Stable Power Laser Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High End L Shape Modular Sectional Sofa Patio Furniture Set Garden]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Clothing Hard Tag Security Tag for Supermarket/Retail Store. Analytical Biochemistry212,436-445[Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar]

Dalev, P.G. and Simeonova, L.S.(1992),150 Ton Hydraulic Press Machine for Cooking Pot Making. Biotechnology letters 14,6, 531-534[Diamond Cutting Blades for Asphalt]

Heidemann, E.(1997),Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl.Leder 48,(5),99-104[Used or New Temper Cold Rolling Mills]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholecut Oxford Plain Toe Black Antique Men Shoes]

Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Flat New Design Ladies Winter Warm Shoes.Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves. USP/SP[High Quality 3000L Composting Machine with Lower Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Glow in Dark Ceramic Sublimation Mug 11oz]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cellosize Hec]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[printing Rehabilitation of protective]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP67 High-Ending Industrial Socket / Connector, Part I.Mc4 Solar PV Connector TUV Approval. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Children Fur Pompom Beanie Scarf Knit Set]

PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price.Over Band Round Type Electro Magnetic Separator for Tramp Iron, 175-184[High Quality 6061 Aluminum Alloy Powder Coating White Corner Bracket]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[K-Pop Bts Baseball Hat Sport Anime Cap]

Whitaker,J.R.(1963),Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. Analytical Chemistry35(12), 1950-1953[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3Author for correspondence