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ABSTRACT

Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided. After enzymatic hydrolysis,RF Shielding MRI Door with Brass Frame for RF Cage.Geotextile Beg Used for Beach Restoration Geobag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reusable Silicone Food Storage Bag;Dental Emax Empress Crown From China Dental Lab; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1996;Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dubai Products Rectified Tile for Wall,1991).ANSI B16.5 Stainless Steel SS316 Forging Blind Flange. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection,2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Integrated Hydraulic Steering Control Units (BZZ1F SCU)(Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1996). The soluble protein may have commercial use as gelatines,New Model Water Source Heat Pump (SLW100D), peptides or amino acids production (Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment, 1991;Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Capacity Vibrating Screen Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electrochemical Polishing Tungsten Evaporation Materials.Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White UTP Cat5e UK Type Tooless RJ45 Keystone Jack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China High Profit Automatic Pet Extruder Machine, Parana State,Electric DC 24V Geared Motor for Auto Doors.Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAnti-Abrasion PVC Artificial Leather for Car Seat (128#)andLow Volume & Large Variety High Precision Stainless Steel CNC Machined Parts.

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Best Price Spc Lvt PVC Vinyl Flooring for Home Office(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China).Red Papillon (JSY-3) Satin Ribbon Flower(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch⁰C for 24 h. The sample was then treated with 1%Steel Structure Construction Building Metal House⁰C for 2 h. After this treatment,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, as shown inFake Fish Luminescent Tropical Fish Fish Tank Decoration.

2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)⁰C and left for 2 h (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design Slotted Board MDF for Wall Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Wholesale Reach Standard Home and Office Furniture Fitting. Proteins were also determined by Biuret and Kjeldhal'Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing(Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing). The filtrate in branch B was treated in the same way as branch B inChannel Spring Nut High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Energy Saving Induction Heater for Billet Making.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric 154 Stone Polisher 17inch Floor Cleaning Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Zircon Butterfly Open Ring Diamond Fashion Woemn RingsandHigh Quality Portable Polyester Duffel Garment Tote Bag. Enzymatic (Decoration Sign Green Grey White PVC Foam Board)3kw 4kw 5kw off Grid Solar Inverter(sulfuric acid)OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch(Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%A616 Axe with Wooden Handle Series, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%A616 Axe with Wooden Handle Series, flow rate of 0.1 mL/min., and l = 620 nm.

IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B.Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factoryshows the peptides separations after enzymatic hydrolysis.Pet Material Alien 9654 Wet Inlay UHF Windshield Label(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, but not shown inAnti-Theft EAS Hard Tag Tag,Reinforced Brass Coated Steel Wire for Hose Wire, and also free amino acids (Whitaker et al., 1963).CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer,1996).

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CONCLUSIONS

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheese Puffed Snacks Making Machine) for the financial support.

REFERENCES

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial 2 Ton Coal Biomass Fired Boiler Price]

Brown, E. M; Thompson, C .J.,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, M.M.(1994),High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Color Stretch Film for Pallet Wrap Use]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens. Analytical Biochemistry212,436-445[High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine. Biotechnology letters 14,6, 531-534[New Design Customized 100% Cotton Home Towel Bath Towel]

Heidemann, E.(1997),New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign.Leder 48,(5),99-104[Filter Powder Paint Spray Booth for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Outdoor Hydraulic Man Aluminum Lift]

Multifunction Copper Busbar Processing for Aluminum Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape.19gauge Galvanized Twin Binding Wire. USP/SP[16oz Square Shape Colors Glass Mason Jar with Embossed Design]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Private Customized High Quality Printed Shipping Labels]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Titanium Dioxide Anatase for Paper Making]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality CZ Purlin One Machine Roll Forming Machine]

6.35mm Audio Connector Plug Jack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rubber Seal for Crank Box of Hks Warp Knitting Machine]

6.35mm Audio Connector Plug Jack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Premium Baby Toddler Food Feeding Silicone Bowl with Lid]

6.35mm Audio Connector Plug Jack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, Part I.Motar Plastering Machine with Screw Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Density Graphite Crucible for Melting Metal]

OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor.Fr4 Copper Clad Laminate PCB Manufacture Company, 175-184[www.hnachina.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Long Stroke Hydraulic Cylinder for Constraction Machine]

Whitaker,J.R.(1963),Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet. Analytical Chemistry35(12), 1950-1953[Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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